The early diagnosis and monitoring of cancers are key factors in effective cancer treatment. Particularly, the separation of biomolecules is an essential step for both diagnostic and analytical purposes. However, the current techniques used to isolate biomolecules are intensive, laborious, and require multiple instruments as well as repeated sample preparations to separate each biomolecule. Thus, an efficient separation system that can simultaneously separate biomolecules from scarce samples is highly desirable. Hence, in this study, we developed a biosilica-based syringe filtration system for the efficient separation of biomolecules from cancer samples using amine-modified diatomaceous earth (AD) with dimethyl 3,3′-dithiobispropionimidate (DTBP). The syringe filter can be an efficient and rapid tool for use in various procedures without complex instruments. The DTBP-based AD system was combined with the syringe filter system for nucleic acid and protein separation from various cancer cells. We demonstrated the efficacy of the DTBP-based AD in a single-filter system for the efficient separation of DNA and proteins within 40 min. This DTBP-based AD syringe filter system showed good rapidity, efficiency, and affordability in the separation of biomolecules from single samples for the early diagnosis and clinical analysis of cancers.

癌症的早期诊断和监测是有效治疗癌症的关键因素。特别是，生物分子的分离是诊断和分析目的的必要步骤。然而，目前用于分离生物分子的技术是密集、费力的，并且需要多台仪器以及重复的样品制备来分离每个生物分子。因此，非常需要一种能够同时从稀有样品中分离生物分子的高效分离系统。因此，在本研究中，我们开发了一种基于生物二氧化硅的注射器过滤系统，使用含 3,3'-二硫代双丙亚氨酸二甲酯 (DTBP) 的胺改性硅藻土 (AD) 有效分离癌症样品中的生物分子。注射器过滤器可以是一种高效、快速的工具，无需复杂的仪器即可用于各种程序。基于 DTBP 的 AD 系统与注射器过滤系统相结合，用于从各种癌细胞中分离核酸和蛋白质。我们证明了基于 DTBP 的 AD 在单过滤器系统中的功效，可在 40 分钟内有效分离 DNA 和蛋白质。这种基于 DTBP 的 AD 注射器过滤器系统在从用于癌症早期诊断和临床分析的单一样本。