Abstract

Age-related changes to the gastrointestinal tract (GIT) can impact how food is digested. Studying the effects of these changes can help identify functional foods for older adults. Cheese was digested using two simulated gastrointestinal in vitro digestion (SGID) models representing adult and elderly gastro-intestinal conditions. Antioxidant capacity was measured using DPPH, FRAP and TPC assays. The ability of cheese to inhibit digestive enzymes was determined by the α-glucosidase and lipase inhibition assays. Digestive aging influenced the bioactivity of cheese, as elderly digestates had significantly lower (p < 0.05) antioxidant, α-glucosidase and lipase inhibitory properties compared to adult digestates. However, soft cheese (feta, goats’, brie) demonstrated greatest potential with comparable radical scavenging properties and lipase inhibition, greatest FRAP and α-glucosidase inhibitory potential. Despite age-related changes, the bioactive properties of cheese were evident following digestion with an older adult SGID model, suggesting cheese has potential as a functional food for older adults.

摘要

胃肠道 (GIT) 的年龄相关变化会影响食物的消化方式。研究这些变化的影响可以帮助确定适合老年人的功能性食品。使用代表成人和老年人胃肠道疾病的两种模拟胃肠体外消化 (SGID) 模型消化奶酪。使用 DPPH、FRAP 和 TPC 测定法测量抗氧化能力。奶酪抑制消化酶的能力由α-葡萄糖苷酶和脂肪酶抑制试验确定。消化老化影响奶酪的生物活性，因为老化的消化物显着降低（p < 0.05) 与成人消化物相比具有抗氧化、α-葡萄糖苷酶和脂肪酶抑制特性。然而，软奶酪（羊乳酪、山羊奶酪、布里干酪）表现出最大的潜力，具有可比的自由基清除特性和脂肪酶抑制作用，最大的 FRAP 和 α-葡萄糖苷酶抑制潜力。尽管与年龄相关的变化，奶酪的生物活性特性在用老年人 SGID 模型消化后很明显，这表明奶酪具有作为老年人功能性食品的潜力。