Abstract Five paraffin-degrading bacteria were isolated from petroleum-contaminated soil collected from Liaohe Oilfield, from which a dominant strain named SS13 was screened out via D2O-labeled single-cell Raman spectroscopy. The dominant strain was identified as Stenotrophomonas maltophilia, and its potential effect in the biodegradation of crude oil was investigated. Results show that the strain produces a biosurfactant which has an emulsification effect on liquid paraffin, and the emulsification index is 65%. The crude oil after incubation with SS13 shows the rates of the wax removal and viscosity reduction reach 74.36% and 47.83%, respectively.

中文翻译：

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优势菌处理原油脱蜡降粘的测定

摘要 从辽河油田采集的石油污染土壤中分离出5种石蜡降解菌，通过D2O标记的单细胞拉曼光谱从中筛选出优势菌株SS13。优势菌株被鉴定为嗜麦芽窄食单胞菌，并研究了其在原油生物降解中的潜在作用。结果表明，该菌株产生的生物表面活性剂对液体石蜡具有乳化作用，乳化指数为65%。原油与SS13孵育后的脱蜡率和降粘率分别达到74.36%和47.83%。