Bísaro pig (BP), like many other local breeds reared in extensive non‐industrial systems, faces many constraints to comply with the EU welfare regulation, particularly regarding the restrictions to surgical castration. In order to adapt an immunocastration protocol to overtake this issue, a puberty timeline is needed. Using gonadal morphometry data from 91 young male BP, this study intended to characterize testicular development, describing the prominent cell types and structures, to ultimately assess the age at puberty in male BP through a mixed prediction model. As expected, the relations between several macro and microscopic parameters and their relation with age were as described within the literature. Post‐pubertal animals have larger and heavier gonads, lower Sertoli cell density/tubule, higher Leydig cell density and larger seminiferous tubules. Meiosis was firstly seen in 44‐day‐old animals, elongated spermatids in 70‐day‐old animals. Complete spermatogenesis was firstly identified in a 90‐day‐old animal. Spermatozoa were present in the epididymis of 23 animals, aged from 70 to 240 days old, and in the vas deferens of 14 animals (105 to 240 days old). The prediction model inferred that male Bísaro pigs reach puberty between 14 and 17 weeks (3 and 4 months old) and become sexually capable from 15 to 19 weeks (3.5 and 4.4 months old). These parameters confirm the sexual precocity of this breed.

中文翻译：

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性腺宏观和微观形态的评估揭示了比萨罗猪的性早熟。

像许多其他在广泛的非工业系统中饲养的本地犬种一样，毕萨罗猪（BP）面临许多限制，难以遵守欧盟的福利法规，特别是在手术cast割方面。为了使免疫cast割方案适应这一问题，需要青春期时间表。本研究使用来自91位年轻男性BP的性腺形态学数据，旨在描述睾丸发育的特征，描述突出的细胞类型和结构，以通过混合预测模型最终评估男性BP的青春期年龄。不出所料，一些宏观和微观参数之间的关系及其与年龄的关系如文献所述。青春期后的动物性腺更大，更重，Sertoli细胞密度/小管更低，Leydig细胞密度更高，生精小管更大。减数分裂首先见于44天大的动物，而精子长于70天大的动物。首次在90天大的动物中鉴定出完全的精子发生。精子存在于23只动物的附睾中，年龄在70至240天大，而输精管则存在于14只动物（105至240天大）中。该预测模型推断，雄性比萨罗猪在14至17周（3至4个月大）达到青春期，并在15至19周（3.5至4.4个月大）具有性能力。这些参数证实了该品种的性早熟。年龄在70到240天之间，并存在于14只动物的输精管中（105到240天大）。该预测模型推断，雄性比萨罗猪在14至17周（3至4个月大）达到青春期，并在15至19周（3.5至4.4个月大）具有性能力。这些参数证实了该品种的性早熟。年龄在70到240天之间，并存在于14只动物的输精管中（105到240天大）。该预测模型推断，雄性比萨罗猪在14至17周（3至4个月大）达到青春期，并在15至19周（3.5至4.4个月大）具有性能力。这些参数证实了该品种的性早熟。