Agarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient (Kav) values measured by gel filtration chromatography. Further, higher Kav values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high‐resolution chromatography with a controllable separation range as beneficial for downstream process.

中文翻译：

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生物分离用琼脂糖微球制造过程中孔结构的调控


通过改变琼脂糖类型和交联度制备了具有可控孔结构的琼脂糖微球。各种琼脂糖可以调节微球基质的凝胶形成，从而影响最终的孔结构。微球中的小孔可以通过具有较高分子量的琼脂糖来制造，这通过凝胶过滤色谱法测量的具有较低分配系数（Kav）值的填充柱来证明。此外，较高的Kav值还表明，随着琼脂糖微球交联度的增加，形成了更多、更大的孔。无论是使用高分子量的琼脂糖还是提高交联度，最终都会导致填充柱流动性能中流速的提高，从而提高分离效率。这为具有可控分离范围的高分辨率色谱提供了基础，有利于下游过程。