当前位置：
X-MOL 学术
›
Biotechnol. Appl. Bioc.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A new potentiometric platform: Antibody cross-linked graphene oxide potentiometric immunosensor for clenbuterol determination
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-24 , DOI: 10.1002/bab.2036 Ebru Birlik Özkütük 1 , Arzu Ersöz 2, 3 , Deniz Uğurağ 1 , Filiz Yılmaz 2 , Rıdvan Say 4
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-24 , DOI: 10.1002/bab.2036 Ebru Birlik Özkütük 1 , Arzu Ersöz 2, 3 , Deniz Uğurağ 1 , Filiz Yılmaz 2 , Rıdvan Say 4
Affiliation
|
An immunosensor is based on the signal measurement obtained upon the reaction of an antibody antigen complex. It plays a significant role in various fields such as environmental analysis, production monitoring, drug detection or screening, veterinary medicine, and animal food. In this study, an antibody crosslinked graphen oxide (GO)-based potentiometric sensor has been developed for fast, simple, and economical detection of clenbuterol. In this context, the photosensitive amino acid bound GO platform is synthesized and used for the preparation of electrode material. Then, polymeric structure is characterized by infrared spectroscopy, and the performance of immunonano platform prepared by potentiometric sensor is evaluated. The effect of pH, response time, selectivity, and sensitivity is investigated. Under the optimized conditions, a simple and rapid method for the determination of clenbuterol from milk sample is established by immuno-potentiometric sensor. The detection limit has found to be 0.87 × 10−9 mmol L−1 for this immuno-potentiometric sensor. This immuno-potentiometric sensor has optimum pH at 7.0, a wide linear response (1.0 × 10−2 to 1.0 × 10−9 mmol L−1), rapid response time (2 Min) and 36 weeks operational lifetime.
中文翻译：
一种新的电位测量平台：抗体交联氧化石墨烯电位免疫传感器用于盐酸克伦特罗的测定
免疫传感器基于在抗体抗原复合物反应时获得的信号测量。它在环境分析、生产监测、药物检测或筛选、兽药和动物食品等各个领域发挥着重要作用。本研究开发了一种基于抗体交联氧化石墨烯 (GO) 的电位传感器，用于快速、简单、经济地检测瘦肉精。在此背景下，合成了光敏氨基酸结合的 GO 平台并用于电极材料的制备。然后，通过红外光谱对聚合物结构进行表征，并对电位传感器制备的免疫纳米平台的性能进行评估。研究了 pH、响应时间、选择性和灵敏度的影响。在优化的条件下，建立了一种简单、快速测定牛奶样品中瘦肉精的方法。检测限为 0.87 × 10-9 mmol L -1用于这种免疫电位传感器。这种免疫电位传感器具有 7.0 的最佳 pH 值、宽线性响应（1.0 × 10 -2至 1.0 × 10 -9 mmol L -1）、快速响应时间（2 分钟）和 36 周的使用寿命。
更新日期：2020-09-24
中文翻译：
一种新的电位测量平台：抗体交联氧化石墨烯电位免疫传感器用于盐酸克伦特罗的测定
免疫传感器基于在抗体抗原复合物反应时获得的信号测量。它在环境分析、生产监测、药物检测或筛选、兽药和动物食品等各个领域发挥着重要作用。本研究开发了一种基于抗体交联氧化石墨烯 (GO) 的电位传感器，用于快速、简单、经济地检测瘦肉精。在此背景下，合成了光敏氨基酸结合的 GO 平台并用于电极材料的制备。然后，通过红外光谱对聚合物结构进行表征，并对电位传感器制备的免疫纳米平台的性能进行评估。研究了 pH、响应时间、选择性和灵敏度的影响。在优化的条件下，建立了一种简单、快速测定牛奶样品中瘦肉精的方法。检测限为 0.87 × 10-9 mmol L -1用于这种免疫电位传感器。这种免疫电位传感器具有 7.0 的最佳 pH 值、宽线性响应（1.0 × 10 -2至 1.0 × 10 -9 mmol L -1）、快速响应时间（2 分钟）和 36 周的使用寿命。
京公网安备 11010802027423号