Inducible DNA Polymerase Chain Reaction Triggered by Oxidative Species,Asian Journal of Organic Chemistry

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Inducible DNA Polymerase Chain Reaction Triggered by Oxidative Species
Asian Journal of Organic Chemistry ( IF 2.8 ) Pub Date : 2020-09-23 , DOI: 10.1002/ajoc.202000377
Sheng Wang ₁ , Jizhong Zhao ₁ , Li Wang ₂ , Jiwen Zhang ₁ , Hongmei Hu ₁ , Ping Yu ₁ , RUI WANG ₃

Affiliation

We report here a chemically synthetic method to introduce a PinB‐modified thymidine into well‐defined DNA first. The modification was designed to disturb the T−A base‐pairing, thus regulate the DNA activity for PCR amplification. By treatment with small molecules, i. e., reactive oxygen species, the DNA polymerase activity was successfully restored. Due to tight temporal regulation and cell‐membrane permeability, our approach could lead to application potential in live cell.

中文翻译：

氧化性物种引发的诱导型DNA聚合酶链反应

我们在这里报告一种化学合成方法，首先将PinB修饰的胸腺嘧啶脱氧核糖核酸引入明确定义的DNA。该修饰旨在干扰T-A碱基配对，从而调节PCR扩增的DNA活性。通过用小分子处理，i。例如活性氧，DNA聚合酶活性被成功恢复。由于严格的时间调节和细胞膜通透性，我们的方法可能会导致在活细胞中的应用潜力。

更新日期：2020-09-23

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