Tarchonanthus Camphoratus L. is traditionally known for its various medicinal purposes. In this study, the T. camphoratus essential oil (TCEO) was isolated via steam distillation, and its chemical constituents were determined using GC–MS. The in vitro antiproliferative effects of TCEO on A549, HepG2, MCF-7 cancer cells, and HUVEC non-tumor cells was investigated using an MTT assay. Flow cytometry analysis was conducted to evaluate cell cycle distribution using propidium iodide staining, and cell death mode using Annexin V-FITC/PI assays. The expression of some apoptosis related genes was investigated using qRT-PCR. Major constituents of TCEO included fenchol, borneol, 3-cyclohexene-1-methanol and 3-ethyl-3-methyl. Cell viability test showed that TCEO is highly effective against MCF-7 cells with IC₅₀ 12.5 µg/mL. Cell cycle arrest at the G1/S phase, and apoptosis mediation were evident in the presence of TCEO. Gene expression analysis of several pro-apoptotic and anti-apoptotic genes revealed the initiation of apoptosis in TCEO-MCF-7 cells. In conclusion, our study confirms the antiproliferative activity of the T. camphoratus essential oil.

Tarchonanthus Camphoratus L. 传统上因其多种药用用途而闻名。在本研究中，通过水蒸气蒸馏分离樟脑精油（TCEO），并使用 GC-MS 测定其化学成分。使用 MTT 测定研究 TCEO 对 A549、HepG2、MCF-7 癌细胞和 HUVEC 非肿瘤细胞的体外抗增殖作用。使用碘化丙啶染色进行流式细胞术分析以评估细胞周期分布，并使用膜联蛋白 V-FITC/PI 测定法评估细胞死亡模式。使用qRT-PCR研究一些凋亡相关基因的表达。TCEO的主要成分包括苯酚、冰片、3-环己烯-1-甲醇和3-乙基-3-甲基。细胞活力测试表明，TCEO 对 MCF-7 细胞非常有效，IC ₅₀ 12.5 µg/mL。TCEO 存在时，细胞周期停滞在 G1/S 期，并且细胞凋亡介导很明显。几个促凋亡和抗凋亡基因的基因表达分析揭示了 TCEO-MCF-7 细胞凋亡的启动。总之，我们的研究证实了樟树精油的抗增殖活性。