In recent years, blast disease caused by Magnaporthe grisea, an ascomycete fungus is becoming a serious threat to pearl millet crop in India and worldwide. Due to the increase in virulent races of pathogen, blast disease management strategies seemed to be very limited. Hence, unraveling the occurrence of blast isolates across India and understanding their virulence and genetic relatedness using molecular markers are the key objectives of this study. From Farmer's field survey we have evidenced variability in blast pathogen across India by recording 10.6 to 7.9 disease severities. A fair to good variation in cultural and conidial characters were also noticed for 17 field isolates. The identity of 17 isolates was confirmed as Magnaporthe grisea by internal transcribed spacer (ITS) region. Based on 12 host differential virulence reactions, five isolates BgKMg1, BdmMg2, MtgMg11, JprMg16 and JmnMg17 recorded highly susceptible (>5 grade) to nine differentials used in the study. While, host differentials ICMB95444, ICMR06222, ICMR11003, IP21187 and ICMV155 found effective for screening virulence of blast disease. Furthermore, genetic relatedness assessed by ITS, inter simple sequence repeats (ISSR) and simple sequence repeats (SSR) markers produced high degree of polymorphism and was able to distinguish the virulence pattern of 17 isolates that correlated with phenotypic screening. Among markers, clustering of isolates within groups was significantly different with remarkable genetic similarity coefficient and bootstrap values. Overall, these results confirm a significant morphological and genetic variation among 17 isolates, thereby helping to elucidate the virulence of pearl millet blast populations in India that could avoid breakdown of resistance and assist breeding improved pearl millet cultivars.

近年来，由稻瘟病菌（Magnaporthe grisea）引起的稻瘟病（一种子囊真菌）正在对印度乃至全世界的粟米作物构成严重威胁。由于病原体的毒力族增加，因此，疾风疾病的管理策略似乎非常有限。因此，本研究的主要目标是阐明印度各地的原始分离株的发生情况，并利用分子标记了解其毒力和遗传相关性。根据Farmer的实地调查，我们通过记录10.6至7.9的疾病严重程度，证明了印度各地爆炸性病原体的变异性。还发现了17个田间分离株在文化和分生孢子特征上有相当不错的变化。17株分离株被确认为稻瘟病菌通过内部转录间隔区（ITS）区域。基于12种宿主差异毒力反应，对本研究中使用的9种差异表现出高度敏感（> 5级）的5个分离株BgKMg1，BdmMg2，MtgMg11，JprMg16和JmnMg17。同时，发现宿主差异ICMB95444，ICMR06222，ICMR11003，IP21187和ICMV155可有效筛查原始疾病的毒力。此外，通过ITS，简单序列间重复（ISSR）和简单序列重复（SSR）标记评估的遗传相关性产生了高度的多态性，并能够区分与表型筛选相关的17种分离株的毒力模式。在标记之间，组内分离株的聚类显着不同，具有显着的遗传相似系数和自举值。总体，