The Structural properties of Zinc oxide nanoparticles (ZnO-NPs) as well as their antibacterial properties against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa; as well as bacteria that are usually found in the mouth of humans and are related to dental conditions, such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus mutans and Streptococcus sanguinis, are presented in this report.

ZnO-NPs were grown by green synthesis, using the Mexican plant Dysphania ambrosioides known in Mexico as "epazote", which was used by native populations of Mexico as a dewormer, is currently used widely in traditional Mexican cuisine and is rich in organic compounds as flavonoids and terpenes which may favor the synthesis of nanoparticles (NPs).

ZnO-NPs were synthesized by the mentioned technology and were compared with commercial ZnO-NPs as a reference. Synthesized and commercial ZnO-NPs were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray energy dispersive spectroscopy (EDS), Fourier transformed infrared spectroscopy (FTIR) and thermogravimetry (TG).

Antibacterial properties were evaluated using a disc diffusion test (Kirby-Bauer method). The results indicate that ZnO-NPs were synthesized in the size range of 5-30 nm. The presence of the ZnO crystalline phase was identified by high resolution transmission electron microscopy HRTEM and XRD analysis. The commercial ZnO-NPs were in the size range of 15-35 nm.

The antibacterial test indicates that most of the bacterial strains used in this study were sensitive to synthesized and commercial NPs, with Prevotella intermedia being the most sensitive to ZnO-NPs.

氧化锌纳米颗粒（ZnO-NPs）的结构特性及其对金黄色葡萄球菌，表皮葡萄球菌，大肠杆菌和铜绿假单胞菌的抗菌性能；本报告还介绍了人类口腔中常见的与牙齿状况有关的细菌，如放线杆菌，牙龈卟啉单胞菌，中间型细肠杆菌，变形链球菌和血链球菌。

ZnO-NPs是通过绿色合成法生长的，使用墨西哥植物Dysphania ambrosioides在墨西哥被称为“ epazote”，墨西哥本地居民将其用作驱虫剂，目前在墨西哥传统美食中广泛使用，并且富含有机化合物，例如类黄酮和萜烯，可能有助于纳米颗粒（NPs）的合成。

通过上述技术合成了ZnO-NP，并与商业ZnO-NP作了比较。通过X射线衍射（XRD），场发射扫描电子显微镜（FESEM），透射电子显微镜（TEM），X射线能量色散光谱（EDS），傅立叶变换红外光谱（FTIR）对合成和商用ZnO-NP进行了表征和热重分析（TG）。

使用圆盘扩散测试（Kirby-Bauer方法）评估抗菌性能。结果表明，ZnO-NPs的合成范围为5-30 nm。通过高分辨率透射电子显微镜HRTEM和XRD分析鉴定出ZnO晶相的存在。商业ZnO-NP的尺寸范围为15-35nm。

抗菌测试表明，本研究中使用的大多数细菌菌株对合成的和商业化的NPs最为敏感，中间媒介型Prevotella对ZnO-NPs最为敏感。