Abstract Different kinds of acid whey, that is, casein whey, whey from traditional Greek yoghurt production, and concentrated acid whey permeate, were used to evaluate their potential for the synthesis of prebiotic galactooligosaccharides (GOS). Therefore, β-galactosidase from Cryptococcus laurentii was used in the form of a whole cell biocatalyst and characterised with regard to optimum temperature and pH, enzyme stability, substrate affinity, and the influence of various cations. The transgalactosylation activity was compared with that of a commercial preparation of Aspergillus oryzae β-galactosidase. With C. laurentii, high GOS yields of 34.6% to 36.1% could be obtained in acid whey, while the yield with A. oryzae ranged from 8.8% to 19.6%. Using C. laurentii, the type of whey had an influence on GOS composition, because the enzyme is able to use galactose, which is present in Greek yoghurt whey, as an acceptor substrate.

中文翻译：

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劳伦隐球菌和米曲霉使用不同酸乳清合成低聚半乳糖

摘要 不同种类的酸性乳清，即酪蛋白乳清、传统希腊酸奶生产的乳清和浓缩酸性乳清渗透液，被用来评估它们合成益生元低聚半乳糖 (GOS) 的潜力。因此，来自 Cryptococcus laurentii 的 β-半乳糖苷酶以全细胞生物催化剂的形式使用，并在最适温度和 pH 值、酶稳定性、底物亲和力和各种阳离子的影响方面进行表征。将转半乳糖基化活性与米曲霉β-半乳糖苷酶的商业制剂的转半乳糖基化活性进行比较。对于 C. laurentii，酸乳清中的 GOS 产率为 34.6% 至 36.1%，而米曲霉的产率为 8.8% 至 19.6%。使用 C. laurentii，乳清的类型对 GOS 成分有影响，