In this study, Fenton oxidation, hydrothermal treatment, and enzymatic hydrolysis were performed in sequence to produce monosaccharide from lignocellulosic biomass. The biomass obtained from each process was washed or unwashed in order that the effect of Fe remaining in the biomass could be investigated further processing. After Fenton oxidation, the Fe remaining in the biomass (washed biomass: 306.74–823.46 ppm, unwashed biomass: 729.58–2456.45 ppm) was found to improve the hydrothermal treatment efficiency and demonstrated degradation rate of 28.94–31.16 % and 34.16–34.75 % in the washed and unwashed biomass, respectively. Hemicellulose was mainly degraded by hydrothermal treatment, which was confirmed by chemical composition and FT-IR analysis. Meanwhile, the Fe remaining in the biomass (washed biomass: 53.38–124.13 ppm, unwashed biomass: 98.25–249.85 ppm) after hydrothermal treatment negatively affected the enzymatic hydrolysis. After 96 h of enzymatic hydrolysis, the cellulose conversion yield differed depending on the condition, with a range of 78.85%–91.08%. The yield was 80.32–91.80 % for washed biomass used in the enzymatic hydrolysis, whereas the yield was low at 78.85–83.53 % for unwashed biomass.

在这项研究中，依次进行Fenton氧化，水热处理和酶促水解，以从木质纤维素生物质生产单糖。洗涤或不洗涤从每个过程获得的生物质，以便可以研究进一步处理中残留在生物质中的Fe的影响。Fenton氧化后，发现生物质中残留的铁（洗涤过的生物质：306.74–823.46 ppm，未洗涤过的生物质：729.58–2456.45 ppm）可以提高水热处理效率，并显示出降解率分别为28.94–31.16％和34.16–34.75％。洗涤过的和未洗涤过的生物质。半纤维素主要通过水热处理降解，这通过化学成分和FT-IR分析证实。同时，残留在生物质中的铁（洗涤过的生物质：53.38–124.13 ppm，未洗涤过的生物质：98。水热处理后25–249.85 ppm）对酶解产生负面影响。酶水解96小时后，纤维素转化率随条件的不同而不同，范围为78.85％–91.08％。用于酶促水解的洗涤过的生物质的产率为80.32–91.80％，而未洗涤过的生物质的产率低至78.85–83.53％。