N⁶-methyladenosine (m⁶A) mRNA methylation varies in response to stress. However, no map of m⁶A mRNA methylation has been obtained for sheep, nor is it known what effect this has on regulating heat stress in sheep. Here, we obtained m⁶A methylation maps of sheep liver tissues with and without heat stress by MeRIP-seq. In total, 8306 m⁶A peaks associated with 2697 genes were detected in the heat stress group, and 12,958 m⁶A peaks associated with 5494 genes were detected in the control group. Peaks were mainly enriched in coding regions and near stop codons with classical RRACH motifs. Methylation levels of heat stress and control sheep were higher near stop codons, although methylation was significantly lower in heat stress sheep. GO and KEGG revealed that differential m⁶A-containing genes were significantly enriched in the stress response and fat metabolism. Our results showed that m⁶A mRNA methylation modifications regulate heat stress in sheep.

N ⁶ -甲基腺苷 (m ⁶ A) mRNA 甲基化随应激而变化。^{然而，尚未获得绵羊的 m 6} A mRNA 甲基化图谱，也不知道这对调节绵羊的热应激有何影响。在这里，我们通过 MeRIP-seq 获得了有和没有热应激的绵羊肝脏组织的 m ⁶ A 甲基化图谱。热应激组共检测到与 2697 个基因相关的8306 m ^{6 A 峰，12958 m 6}在对照组中检测到与 5494 个基因相关的峰。峰主要富集在编码区和具有经典 RRACH 基序的终止密码子附近。热应激和对照绵羊的甲基化水平在终止密码子附近较高，尽管热应激绵羊的甲基化水平显着降低。GO 和 KEGG 揭示了含有差异 m ⁶ A 的基因在应激反应和脂肪代谢中显着富集。我们的研究结果表明，m ⁶ A mRNA 甲基化修饰调节绵羊的热应激。