Calcium signalling plays a fundamental role in fungal intracellular signalling. Previous approaches (fluorescent dyes, bioluminescent aequorin, genetically encoded cameleon probes) with imaging rapid subcellular changes in cytosolic free calcium ([Ca²⁺]_c) in fungal cells have produced inconsistent results. Recent data obtained with new fluorescent, genetically encoded GCaMP probes, that are very bright, have resolved this problem. Here, exposing conidia or conidial germlings to high external Ca²⁺, as an example of an external stressor, induced very dramatic, rapid and dynamic [Ca²⁺]_c changes with localized [Ca²⁺]_c transients and waves. Considerable heterogeneity in the timing of Ca²⁺ responses of different spores/germlings within the cell population was observed.

钙信号在真菌细胞内信号传导中起着重要作用。^{以前对真菌细胞中游离钙 ([Ca 2+} ] _c ) 的快速亚细胞变化进行成像的方法（荧光染料、生物发光水母发光蛋白、基因编码的变色龙探针）产生了不一致的结果。最近使用非常明亮的新型荧光基因编码 GCaMP 探针获得的数据解决了这个问题。在这里，将分生孢子或分生孢子幼体暴露于高外部 Ca ²⁺中（作为外部应激源的一个例子）会引起非常显着、快速和动态的 [Ca ²⁺ ] _c变化，局部 [Ca ²⁺ ] _c瞬态和波。观察到细胞群内不同孢子/胚芽的Ca ²⁺响应时间存在相当大的异质性。