We investigated the presence of carbapenemases in carbapenem-resistant Pseudomonas aeruginosa isolates, which were collected over a 14-month period in a Turkish hospital, with in-depth molecular characterization of carbapenemase-producing isolates. Among 45 study isolates, 2 isolates were identified as carbapenemase producers by both Carba NP and Carbapenem Inactivation Method tests, and only 1 of them gave a positive result in polymerase chain reaction tests for a carbapenemase gene (bla_VIM). Whole genome sequencing of the 2 isolates revealed the presence of bla_VIM-5 gene in an ST308 isolate, while the other one expressed IMP-7 in an ST357 isolate; both STs are considered high-risk clones. The 2 carbapenemase-producing isolates were multidrug resistant, as they harbored other resistance determinants, including a variant of the recently described plasmid-encoded fluoroquinolone resistance determinant crpP gene, crpP-2. We report for the first time P. aeruginosa high-risk clones carrying VIM-5– and IMP-7–type carbapenemases with multiple resistance determinants in Turkey.

我们调查了碳青霉烯酶耐药铜绿假单胞菌菌株中碳青霉烯酶的存在，这些菌株在土耳其医院经过14个月的时间收集，并对产生碳青霉烯酶的菌株进行了深入的分子鉴定。在45个研究菌株中，通过Carba NP和Carbapenem灭活方法测试均鉴定出2个菌株为碳青霉烯酶生产者，其中只有1个在碳青霉烯酶基因（bla _VIM）的聚合酶链反应测试中给出了阳性结果。2个分离株的全基因组测序揭示了bla _VIM-5的存在该基因在ST308分离株中表达，而另一个在ST357分离株中表达IMP-7。两个ST均被视为高风险克隆。该碳青霉烯酶2生产菌株多重耐药性的，因为它们窝藏其他抗性决定因素，包括最近描述质粒编码的氟喹诺酮抗性决定的变体CRPP基因，CRPP-2 。我们在土耳其首次报道了携带VIM-5和IMP-7型碳青霉烯酶和多种抗性决定簇的铜绿假单胞菌高风险克隆。