Granulation of halophilic activated sludge is an important solution to solve the problem of solid-liquid separation in biological treatment of saline wastewater. This study demonstrated that by adding divalent cations into the saline influent with low organic load, halophilic granular sludge with an average diameter of 910 ± 10 μm can be cultivated. The close correlation between divalent cations and particle size indicated that Ca²⁺ played a major role in the granulation process. Ca²⁺ was accumulated in halophilic granular sludge, which provided an inorganic carrier for microbial aggregation and leaded to the dominance of halophilic bacteria of the family Flavobacteriaceae. The halophilic bacteria secreted a large amount of extracellular polymeric substances (EPS), which contained 70.0 ± 0.02% protein. By enhancing the EPS network of protein and Ca²⁺, halophilic granular sludge was formed. The addition of Mg²⁺ enhanced the network of Mg²⁺ and loosely bound EPS, which could be destroyed due to Na⁺ substitution. This study provides an effective granulation method for halophilic activated sludge.

嗜盐活性污泥的颗粒化是解决含盐废水生物处理中固液分离问题的重要解决方案。该研究表明，通过向有机负荷低的盐水进水中添加二价阳离子，可以培养出平均直径为910±10μm的嗜盐颗粒污泥。二价阳离子与粒径之间的密切关系表明，Ca ²⁺在制粒过程中起主要作用。钙²⁺细菌在嗜盐颗粒污泥中积累，这为微生物聚集提供了无机载体，并导致黄杆菌科嗜盐细菌占主导地位。嗜盐细菌分泌大量细胞外聚合物（EPS），其中包含70.0±0.02％的蛋白质。通过增强蛋白质和Ca ²⁺的EPS网络，形成了嗜盐颗粒污泥。添加Mg ²⁺增强的Mg的网络²⁺和松散结合的EPS，这可能被破坏，由于钠⁺取代。该研究为嗜盐活性污泥提供了一种有效的制粒方法。