In recent years, the stimuli-responsive delivery of drugs based on diselenide-bridged polymeric nanocomposites have gathered significant attention from researchers towards augmented anticancer therapy, owing to the unique dual reactive oxygen species (ROS) as well as glutathione (GSH) redox-responsive diselenide bonds. Despite their success in delivery of diverse therapeutics, the fabrication of such diselenide-bridged polymeric nanocomposites with high performance efficiency is often limited due to the relatively high sensitivity of diselenide bonds to various stimuli such as light, temperature, and both oxidation and reduction. In an attempt to address these attributes, herein, we demonstrate the fabrication of indocyanine green (ICG)-loaded diselenide-containing polymeric nanoparticles using the supercritical fluid (SCF)-assisted rapid and facile synthesis approach for ROS/GSH-responsive drug delivery platform towards augmented anticancer therapy. Initially, the diselenide-containing poly (ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) block copolymers (PSe) are synthesized based on stannous octoate initiated ring-opening polymerization and subsequent esterification with carboxylic acid-functionalized polyethylene glycol (PEG-COOH). Further, the ICG molecules are loaded into the copolymer nanocomposites via the incorporation of coprecipitation interactions between PSe and ICG through the convenient and highly effective SCF technology. These nanocomposites afforded high drug loading (up to 48.5%) and encapsulation efficiency (up to 85.1%), along with uniform distribution and desired photo-stability. Finally, various photodynamic therapy (PDT)-related experiments both in vitro and in vivo have shown that the fabricated ROS-responsive drug delivery system based on ICG and PSe is capable of devastating tumor cells.

近年来，由于独特的双重活性氧（ROS）以及谷胱甘肽（GSH）的氧化还原反应性，基于二硒化物桥接的聚合物纳米复合材料的刺激反应性药物已引起研究人员对增强抗癌治疗的关注。二硒键。尽管它们成功地递送了多种疗法，但是由于二硒键对各种刺激（例如光，温度以及氧化和还原）的相对较高的敏感性，因此具有高性能效率的这种二硒键桥接的聚合物纳米复合材料的制造常常受到限制。为了尝试解决这些属性，我们展示了使用超临界流体（SCF）辅助的ROS / GSH响应药物传递平台的快速，简便合成方法，向吲哚菁绿（ICG）加载含二硒化物的聚合物纳米粒子的制备，以增强抗癌治疗。最初，基于辛酸亚锡引发的开环聚合反应，然后用羧酸官能化的聚乙二醇酯化，合成含二硒化物的聚（乙二醇）-聚（ε-己内酯）-聚（乙二醇）嵌段共聚物（PSe） （PEG-COOH）。此外，将ICG分子装入共聚物纳米复合材料中 基于辛酸亚锡引发的开环聚合反应，然后用羧酸官能化的聚乙二醇（PEG）酯化合成含二硒化物的聚（乙二醇）-聚（ε-己内酯）-聚（乙二醇）嵌段共聚物（PSe） -COOH）。此外，将ICG分子装入共聚物纳米复合材料中 基于辛酸亚锡引发的开环聚合反应，然后用羧酸官能化的聚乙二醇（PEG）酯化合成含二硒化物的聚（乙二醇）-聚（ε-己内酯）-聚（乙二醇）嵌段共聚物（PSe） -COOH）。此外，将ICG分子装入共聚物纳米复合材料中通过便捷高效的SCF技术，将PSe和ICG之间的共沉淀相互作用纳入其中。这些纳米复合材料具有较高的载药量（高达48.5％）和包封效率（高达85.1％），以及均匀的分布和所需的光稳定性。最后，在体外和体内进行的各种与光动力疗法（PDT）相关的实验均表明，基于ICG和PSe制成的ROS反应药物递送系统能够破坏肿瘤细胞。