Zinc (Zn²⁺) is an essential micronutrient that is required for a wide variety of cellular processes. Tools and methods have been instrumental in revealing the myriad roles of Zn²⁺ in cells. This review highlights recent developments fluorescent sensors to measure the labile Zn²⁺ pool, chelators to manipulate Zn²⁺ availability, and fluorescent tools and proteomics approaches for monitoring Zn²⁺-binding proteins in cells. Finally, we close with some highlights on the role of Zn²⁺ in regulating cell function and in cell signaling.

中文翻译：

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阐明锌细胞生物学的工具和技术

锌（Zn ²⁺）是多种细胞过程所需的必需微量营养素。工具和方法有助于揭示细胞中Zn ²⁺的多种作用。这篇综述着重介绍了荧光传感器来测量不稳定的Zn ²⁺库，螯合剂以控制Zn ^2+的可利用性以及荧光工具和蛋白质组学方法的最新发展，这些工具可监测细胞中Zn ^2+的结合蛋白。最后，我们以Zn ²⁺在调节细胞功能和细胞信号传导中的作用作为重点。