Abstract This study aimed to determine the heat processing impact on the protein molecular structure (amide I, amide II, α-helix, and β-sheet), and the nutritional characteristics (nutrient profiles, rumen degradation, intestinal digestion, and true protein supply) of faba bean grown in western Canada. Low tannin (LT) and normal tannin (NT) faba bean varieties were used for the present study, where varieties (n = 3) for each tannin level were used as a replicate. Based on previously published studies, sample groups were either heated with steam pressure at 121 °C for 30 minute (min) using a medium steam sterilizer or with microwave irradiation for 3 min in a conventional microwave with a power of 900 W and irradiation frequency of 2450 MHz. All seed was ground using a roller mill before heating. Heat-induced molecular structure changes were determined with Fourier Transformed Infrared spectroscopy (FTIR) while nutrient profiles, including dry matter (DM), crude protein (CP), soluble crude protein (SCP), and starch were obtained with traditional chemical analyses. An in situ rumen degradation study and a modified three-step in vitro technique was performed to analyze the degradation parameters and intestinal digestibility of feed nutrients in faba bean. Results showed that the peak area ratio of amide I to amide II was lower in normal tannin faba bean types (1.835 vs. 2.073 absorbance units (AU), P

中文翻译：

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热处理方法对生长在加拿大西部的蚕豆（低单宁和正常单宁）的蛋白质分子结构、理化和营养特性的影响

摘要 本研究旨在确定热处理对蛋白质分子结构（酰胺 I、酰胺 II、α-螺旋和β-折叠）和营养特性（营养成分、瘤胃降解、肠道消化和真正的蛋白质供应）的影响。 ）在加拿大西部种植的蚕豆。本研究使用低单宁 (LT) 和正常单宁 (NT) 蚕豆品种，其中每个单宁水平的品种 (n = 3) 用作重复。根据先前发表的研究，样品组要么使用中等蒸汽灭菌器在 121 °C 的蒸汽压力下加热 30 分钟（分钟），要么在功率为 900 W 且辐照频率为2450 兆赫。所有种子在加热前使用辊磨机研磨。用傅里叶变换红外光谱 (FTIR) 确定热诱导的分子结构变化，而通过传统化学分析获得包括干物质 (DM)、粗蛋白 (CP)、可溶性粗蛋白 (SCP) 和淀粉在内的营养成分。进行了原位瘤胃降解研究和改良的三步体外技术，以分析蚕豆中饲料营养素的降解参数和肠道消化率。结果表明，酰胺 I 与酰胺 II 的峰面积比在正常单宁蚕豆类型中较低（1.835 vs. 2.073 吸光度单位 (AU)，P 进行了原位瘤胃降解研究和改良的三步体外技术，以分析蚕豆中饲料营养素的降解参数和肠道消化率。结果表明，正常单宁蚕豆类型中酰胺 I 与酰胺 II 的峰面积比较低（1.835 vs. 2.073 吸光度单位 (AU)，P 进行了原位瘤胃降解研究和改良的三步体外技术，以分析蚕豆中饲料营养素的降解参数和肠道消化率。结果表明，正常单宁蚕豆类型中酰胺 I 与酰胺 II 的峰面积比较低（1.835 vs. 2.073 吸光度单位 (AU)，P