Abstract

Interaction of slow-wave rhythmic components of cardiac, respiratory and motor activities was analyzed in non-narcotized neonatal (P1) and early postnatal (P16) rat pups under normal conditions and after activation of cholinoreactive structures. A reversible acetylcholinesterase (AChE) inhibitor physostigmine (eserine) and the M-cholinolytic methacin were used in the experiments. It was established that in intact P1 rats intersystemic interactions are mainly realized via oscillations within the near- and multi-minute ranges. Correlative interactions between the rhythms in the decasecond range that were not involved in integrative processes in P1 rats increased significantly at P16. In rat pups of both ages, changes in the intensity and pattern of motor activity (MA) tended to precede the generation of modulatory oscillations of the respiratory and heart rates. AChE inhibition by physostigmine and subsequent activation of cholinoreactive structures evoked a decrease in the level of intersystemic interactions across all ranges of the modulatory rhythms in rats of both ages. The methacin-evoked blockade of peripheral M-cholinoreceptors (M-ChRs) decreased the level of interactions mediated by the near- and multi-minute rhythms at P1 but augmented them at P16. Physostigmine injection at P1 and P16 exerted no significant effect on cardio-somatomotor interactions, with these interactions in P1 rats being maintained by a wider range of the modulatory rhythms. The same situation was also observed during the physostigmine aftereffect period in all interacting systemic pairs. By contrast, after the blockade of peripheral M-ChRs the existing intersystemic interactions were more difficult to be disrupted in P16 rat pups. The activation of cholinoreactive structures, which occurred against the background of M-ChRs blockade, led to disrupt the coordinating interactions in the “MA–respiratory rate” pair. In P1 rats, these functional relationships did not recover, while in P16 rats they were resumed already during the aftereffect period. The power of modulatory rhythms of activity in the systems studied here was not directly related to intersystemic interactions. Changes in intersystemic interactions were due to heterochrony both in the maturation of these functional systems and the involvement of different cholinoreactive structures in the processes of intersystemic regulation.

中文翻译：

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激活胆碱反应性结构后，M-胆碱受体阻滞剂对新生大鼠体内内脏系统间相互作用的影响

摘要

在正常条件下和激活胆碱反应性结构后，对非麻醉新生儿（P1）和出生后早期（P16）的幼仔分析了心脏，呼吸和运动活动的慢波节律成分的相互作用。实验中使用了可逆的乙酰胆碱酯酶（AChE）抑制剂毒扁豆碱（eserine）和M-胆碱分解型甲基苯丙氨酸。已经确定，在完整的P1大鼠中，系统间的相互作用主要是通过在近和数分钟范围内的振荡来实现的。在P16大鼠中，十秒范围内不参与整合过程的节律之间的相关相互作用显着增加。在两个年龄段的幼崽中，运动强度（MA）强度和模式的变化往往先于呼吸频率和心率的调制振荡的产生。毒扁豆碱对AChE的抑制作用以及随后胆碱反应性结构的激活引起了两个年龄段大鼠的所有调节性节律的系统间相互作用水平的降低。甲氧苄啶引起的外周M-胆碱受体（M-ChRs）的阻滞降低了P1处近或多分钟节律介导的相互作用水平，但在P16处增强了相互作用。在P1和P16注射毒扁豆碱对心脏动体运动的相互作用没有显着影响，而在P1大鼠中的这些相互作用则通过更广泛的调节性节律得以维持。在毒扁豆碱后效应期间，在所有相互作用的系统对中也观察到相同的情况。相比之下，在阻断外周M-ChRs之后，现有的系统间相互作用在P16大鼠幼仔中更难被破坏。胆碱反应性结构的激活发生在M-ChRs阻断的背景下，从而破坏了“ MA-呼吸频率”对中的协调相互作用。在P1大鼠中，这些功能关系没有恢复，而在P16大鼠中，它们在后效应期已经恢复。在此研究的系统中，调节性活动节奏的力量与系统间的交互作用并不直接相关。