Genotoxic potential of herbicide bispyribac-sodium was evaluated in fish Clarias batrachus using micronucleus (MN) test and comet assay. Fish were exposed to three environmentally relevant test concentrations of the herbicide for 20, 25 and 30 days. Significant effects (p < 0.05) for both concentration and duration of exposure were observed in herbicide exposed fish. Similar trend of DNA damage was observed through MN test and comet assay. Maximum DNA damage was observed in fish exposed to highest concentration of herbicide at all duration. Maximum damage was observed on day 25 at all concentrations followed by a decline. This study established C. batrachus as an ecotoxicological model for bispyribac-sodium induced genotoxicity testing. It further confirmed that both MN test and comet assay are useful tool for assessment of genotoxicity induced by water pollutants.

除草剂双嘧苯甲酸钠的潜在遗传毒性在鱼评价胡子蟾使用微核（MN）测试和彗星试验。将鱼暴露于三种与环境有关的试验浓度的除草剂中20、25和30天。 在暴露于除草剂的鱼中，观察到浓度和持续时间的显着影响（p <0.05）。通过MN试验和彗星试验观察到相似的DNA损伤趋势。在所有时间内，暴露于最高浓度的除草剂的鱼体内最大的DNA损伤被观察到。在所有浓度下，在第25天观察到最大的破坏，然后下降。这项研究建立了巴氏梭菌作为双嘧啶钠诱导的基因毒性测试的生态毒理模型。它进一步证实，MN试验和彗星试验都是评估水污染物引起的基因毒性的有用工具。