Phosphatidate phosphatase catalyzes the penultimate step in the synthesis of triacylglycerol and regulates the synthesis of membrane phospholipids. There is much interest in this enzyme because it controls the cellular levels of its substrate phosphatidate and product diacylglycerol; defects in the metabolism of these lipid intermediates are the basis for lipid-based diseases such as obesity, lipodystrophy, and inflammation. The measurement of phosphatidate phosphatase activity is required for studies aimed at understanding its mechanisms of action, how it is regulated, and for screening its activators and/or inhibitors. Enzyme activity is determined through the use of radioactive and nonradioactive assays that measure the product diacylglycerol or Pi. However, sensitivity and ease of use are variable across these methods. This review summarizes approaches to synthesize radioactive phosphatidate, to analyze radioactive and nonradioactive products diacylglycerol and Pi and discusses the advantages and disadvantages of each phosphatidate phosphatase assay.

中文翻译：

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磷脂酸磷酸酶测定的回顾。


磷脂酸磷酸酶催化三酰甘油合成的倒数第二步并调节膜磷脂的合成。人们对这种酶很感兴趣，因为它控制其底物磷脂酸和产物二酰基甘油的细胞水平。这些脂质中间体的代谢缺陷是肥胖、脂肪营养不良和炎症等脂质疾病的基础。旨在了解其作用机制、其调节方式以及筛选其激活剂和/或抑制剂的研究需要测量磷脂酸磷酸酶活性。酶活性通过使用放射性和非放射性检测来测量产物二酰基甘油或 Pi 来确定。然而，这些方法的灵敏度和易用性各不相同。本综述总结了合成放射性磷脂酸、分析放射性和非放射性产物二酰甘油和 Pi 的方法，并讨论了每种磷脂酸磷酸酶测定的优点和缺点。