One of the major challenges posed by DNA-binding proteins to locate and bind their target sequences is the in vivo cellular environment, which is densely crowded by the high concentrations of other proteins and macromolecules that may hinder the diffusion of a protein molecule searching for its binding site on the DNA. To explore the molecular crowding effect on the target search, in this study we develop a theoretical method based on a discrete-state stochastic ##IMG## [http://ej.iop.org/images/1742-5468/2020/9/093204/jstatabb019d1.gif] framework ##IMG## [http://ej.iop.org/images/1742-5468/2020/9/093204/jstatabb019d2.gif] to explicitly investigate the role of bulk crowding in the cellular environment during the protein search. It is found that based on the spatial positions of the target and the crowders, and the size of the crowders, the protein search dynamics can accelerate or slow down and this is discussed here using...

中文翻译：

---

理论上大量拥挤对DNA结合蛋白的目标搜索动力学的作用。

DNA结合蛋白定位和结合其靶序列所面临的主要挑战之一是体内细胞环境，体内细胞环境被高浓度的其他蛋白质和大分子拥挤，这些蛋白质和大分子分子可能阻碍寻找其蛋白质分子的扩散。 DNA上的结合位点。为了探索分子拥挤对目标搜索的影响，在这项研究中，我们开发了一种基于离散状态随机## IMG ## [http://ej.iop.org/images/1742-5468/2020/ 9/093204 / jstatabb019d1.gif]框架## IMG ## [http://ej.iop.org/images/1742-5468/2020/9/093204/jstatabb019d2.gif]明确调查了批量拥挤在蛋白质搜索过程中的细胞环境。根据目标和拥挤者的空间位置以及拥挤者的大小，发现