Integration of stimulated emission depletion (STED) with digital scanned light-sheet microscopy (DSLM) using a Bessel beam achieves fast, high-resolution and large field of view (FOV) imaging. Here, we report on a combination method of DSLM and STED by using a Bessel beam for excitation and a hollow Gaussian beam for depletion. Owing to the advantages of confined axial distribution around the focal plane for the hollow Gaussian depletion beam, those fluorescence signals from the periphery of the Bessel excitation pattern are depleted by stimulated emission. A super-resolved light sheet with suppressed side lobes is achieved. The numerical simulation results show that the use of the Bessel beam for the excitation and the hollow Gaussian beam for the STED results in a DSLM system with enhanced performance of the axial resolution and image contrast over a moderately large FOV.

中文翻译：

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通过受激发射损耗在数字扫描光片显微镜中的轴向分辨率和对比度增强

使用贝塞尔光束将激发发射损耗（STED）与数字扫描光片显微镜（DSLM）集成在一起，可实现快速，高分辨率和大视野（FOV）成像。在这里，我们通过使用贝塞尔光束激发和空心高斯光束耗尽来报告DSLM和STED的组合方法。由于空心高斯耗尽光束在焦平面周围的轴向分布有限的优点，来自贝塞尔激发图案外围的那些荧光信号被受激发射所耗尽。获得了具有抑制的旁瓣的超分辨光片。