BACKGROUND:DNA methylation balance is an important regulatory mechanism for mammalian and plant development. The fruit ripening and anthocyanin accumulation of Vaccinium corymbosum are complex developmental processes that involve numerous physiological, biochemical, and structural alterations. OBJECTIVE:This study aimed to investigate the correlation of DNA methylation balance, DNA methylation and demethylation-related gene expression models and anthocyanin accumulation during blueberry fruit ripening. METHODS:The anthocyanin contents during V. corymbosum ‘O’Neal’ fruit development were evaluated. The V. corymbosum DNA methylation- and anthocyanin accumulation-related genes were isolated, and their relative expression patterns were detected during flower bud enlargement and fruit development. Moreover, the relative expression patterns of anthocyanin biosynthetic genes and the dynamic changes in the DNA methylation of the promoter sequences of key anthocyanin biosynthetic genes were evaluated. RESULTS:The results showed that the DNA methylation level of V. corymbosum fruit was consistent with anthocyanin accumulation during ripening, and the expression levels of anthocyanin biosynthetic and DNA methylation-related genes. CONCLUSIONS:During V. corymbosum fruit ripening, anthocyanin accumulation is regulated partially by DNA methylation balance of VcCHS and VcANS promoters.

中文翻译：

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Vaccinium corymbosum果实成熟过程中DNA甲基化平衡与花色苷积累有关

背景：DNA甲基化平衡是哺乳动物和植物发育的重要调控机制。越橘的果实成熟和花色苷积累是复杂的发育过程，涉及许多生理，生化和结构改变。目的：研究蓝莓果实成熟过程中DNA甲基化平衡，DNA甲基化和去甲基化相关基因表达模型与花色苷积累的相关性。方法：评估了V. corymbosum'O'Neal'果实发育过程中的花色苷含量。分离了V. corymbosum DNA甲基化和花青素积累相关基因，并在花蕾扩大和果实发育过程中检测了它们的相对表达模式。此外，评价了花色苷生物合成基因的相对表达模式以及主要花色苷生物合成基因启动子序列DNA甲基化的动态变化。结果：结果表明，虎杖果实的DNA甲基化水平与成熟过程中花青素的积累，花青素的生物合成及DNA甲基化相关基因的表达水平一致。结论：Corymbosum果实成熟期间，花色素苷的积累部分受VcCHS和VcANS启动子DNA甲基化平衡的调节。棒茎果实与成熟期间花青素的积累以及花青素生物合成和DNA甲基化相关基因的表达水平一致。结论：Corymbosum果实成熟期间，花色素苷的积累部分受VcCHS和VcANS启动子DNA甲基化平衡的调节。棒茎果实与成熟期间花青素的积累以及花青素生物合成和DNA甲基化相关基因的表达水平一致。结论：Corymbosum果实成熟期间，花色素苷的积累部分受VcCHS和VcANS启动子DNA甲基化平衡的调节。