Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models,Journal of Materials Chemistry B

当前位置： X-MOL 学术 › J. Mater. Chem. B › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-09-23 , DOI: 10.1039/d0tb01969k
Na He _{1,

2,

3,

4,

5} , Yude Wang _{6,

7,

8,

9} , Yan Huang _{6,

7,

8,

9,

10} , Lingxin Chen _{10,

11,

12,

13,

14} , Xiaoyan Wang _{6,

7,

8,

9,

10} , Changjun Lv _{6,

7,

8,

9} , Shouwei Yue _{1,

2,

3,

4,

5}

Affiliation

Acute lung injury (ALI) is a diffuse inflammatory pulmonary damage caused by excessive ROS that break the coordination of normal physiological structures and functions. Hypochlorous acid (HOCl), one kind of ROS, is a hopeful biological marker for inflammation-related diseases. Therefore, the excessive generation of HOCl might be a significant reason for oxidative injury in ALI. Herein, we developed a fluorescent probe, namely BCy-HOCl, for quantitatively monitoring and visualizing HOCl in living cells and in vivo. The probe BCy-HOCl displayed a significant fluorescence signal enhancement towards HOCl with excellent selectivity and sensitivity. The variation of HOCl in the ALI cell model and ALI mouse model was evaluated with BCy-HOCl to clarify the relationship between ALI and HOCl. Our results verified that the HOCl levels conspicuously increased with the severity of the ALI. Thus, HOCl is likely to play a crucial part in the process of ALI, which will probably provide a new strategy for its treatment.

中文翻译：

通过选择性荧光探针检测急性肺损伤细胞和小鼠模型中次氯酸的波动

急性肺损伤（ALI）是由过量的ROS引起的弥散性炎性肺损伤，破坏了正常生理结构和功能的协调。次氯酸（次氯酸）是一种与炎症相关疾病的有希望的生物标志物。因此，HOCl的过量生成可能是ALI氧化损伤的重要原因。在这里，我们开发了一种荧光探针，即BCy-HOCl，用于定量监测和可视化活细胞和体内的HOCl。。探针BCy-HOC1以优异的选择性和灵敏度对HOC1显示出明显的荧光信号增强。用BCy-HOCl评估ALI细胞模型和ALI小鼠模型中HOCl的变化，以阐明ALI与HOCl之间的关系。我们的结果证明，HOCI水平随ALI的严重程度而明显增加。因此，HOC1可能在ALI的过程中起关键作用，这可能为其治疗提供新的策略。

更新日期：2020-10-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11