当前位置：
X-MOL 学术
›
Anal. Methods
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Probing arsenic trioxide (ATO) treated leukemia cell elasticities using atomic force microscopy.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-09-23 , DOI: 10.1039/d0ay01017k Hélène Fortier 1 , Valerie Gies 2 , Fabio Variola 3 , Chen Wang 4 , Shan Zou 5
Analytical Methods ( IF 2.7 ) Pub Date : 2020-09-23 , DOI: 10.1039/d0ay01017k Hélène Fortier 1 , Valerie Gies 2 , Fabio Variola 3 , Chen Wang 4 , Shan Zou 5
Affiliation
|
Conventional analytical techniques allow for the diagnosis of leukemia, blood and bone marrow cancers, as well as their classification into the different subtypes. However, a better understanding of the cancer treatment through cell apoptosis staging is still required. Evaluation of the timeline and responses of acute promyelocytic leukemia (APL) cells to the arsenic trioxide (ATO) treatment is essential for determining the oral dosage in leukemia prognosis. Here, an Atomic Force Microscopy (AFM) indentation approach has been used to evaluate the mechanical responses of cellular responses of APL cells to ATO treatment, alongside well-established cell viability assays, as a novel method to determine the impact of drugs. In addition, cell morphology was quantified to monitor cellular apoptosis. Viability, morphology and elasticity changes of NB4 cells (derived from Human APL patients) were correlated to different time courses of the ATO treatments. Unveiling the relationships among structural, morphological and nanomechanical properties in response to ATO drug treatment promises to pave the way for novel diagnostic tools for drug screening and for a better understanding of the specific physical and biological effects of drugs on diseased cells.
中文翻译：
使用原子力显微镜探测三氧化二砷（ATO）处理的白血病细胞弹性。
传统的分析技术可以诊断白血病，血液和骨髓癌，以及将其分类为不同的亚型。然而，仍然需要通过细胞凋亡分期对癌症治疗有更好的了解。评估时间表和急性早幼粒细胞白血病（APL）细胞对三氧化二砷（ATO）治疗的反应对于确定口服剂量对白血病的预后至关重要。在这里，原子力显微镜（AFM）压痕法已被用来评估APL细胞对ATO处理的细胞反应的机械反应，以及完善的细胞生存力测定法，作为确定药物影响的新方法。另外，量化细胞形态以监测细胞凋亡。可行性，NB4细胞的形态和弹性变化（源自人类APL患者）与ATO治疗的不同时间进程相关。揭示响应ATO药物治疗的结构，形态和纳米机械性能之间的关系，有望为药物筛选的新型诊断工具和更好地了解药物对患病细胞的特定物理和生物学作用铺平道路。
更新日期：2020-10-15
中文翻译：
使用原子力显微镜探测三氧化二砷（ATO）处理的白血病细胞弹性。
传统的分析技术可以诊断白血病，血液和骨髓癌，以及将其分类为不同的亚型。然而，仍然需要通过细胞凋亡分期对癌症治疗有更好的了解。评估时间表和急性早幼粒细胞白血病（APL）细胞对三氧化二砷（ATO）治疗的反应对于确定口服剂量对白血病的预后至关重要。在这里，原子力显微镜（AFM）压痕法已被用来评估APL细胞对ATO处理的细胞反应的机械反应，以及完善的细胞生存力测定法，作为确定药物影响的新方法。另外，量化细胞形态以监测细胞凋亡。可行性，NB4细胞的形态和弹性变化（源自人类APL患者）与ATO治疗的不同时间进程相关。揭示响应ATO药物治疗的结构，形态和纳米机械性能之间的关系，有望为药物筛选的新型诊断工具和更好地了解药物对患病细胞的特定物理和生物学作用铺平道路。
京公网安备 11010802027423号