C-reactive protein (CRP) is a major acute-phase protein, and it is produced by the liver in response to a pro-inflammatory stimulus. Given that human and canine CRP have a similar molecular structure, the assays used for human CRP detection have been used to measure CRP concentrations in dogs. We evaluated the use of a human CRP assay (Biotecnica CRP assay) and validated its application in dogs. We analyzed 91 canine serum samples with a fully automated analyzer. Our validation was based on the evaluation of imprecision, limits of linearity, limits of quantification, and an evaluation of interferences. The new assay was also compared with the Randox CRP assay, a validated assay for the measurement of CRP. Intra- and inter-assay repeatability were <8% and <11%, respectively. The tested assay proportionally measured canine CRP in an analytical range up to 60 mg/L; however, hemoglobin, triglycerides, and bilirubin interfered with the determination. Good agreement, with the presence of proportional systematic bias, was observed between Biotecnica and Randox assays. The Biotecnica CRP assay provides reliable measurement of CRP in canine serum, provided that samples are free of interferents.

C 反应蛋白 (CRP) 是一种主要的急性期蛋白，它是由肝脏响应促炎刺激产生的。鉴于人和犬 CRP 具有相似的分子结构，用于人类 CRP 检测的分析已被用于测量犬的 CRP 浓度。我们评估了人类 CRP 检测（Biotecnica CRP 检测）的使用，并验证了其在狗中的应用。我们使用全自动分析仪分析了 91 份犬血清样本。我们的验证基于对不精密度、线性限制、定量限制和干扰评估的评估。新检测还与 Randox CRP 检测进行了比较，后者是一种经过验证的 CRP 测量检测方法。批内和批间重复性分别<8% 和<11%。测试的测定按比例测量犬 CRP，分析范围高达 60 mg/L；然而，血红蛋白、甘油三酯和胆红素会干扰测定。在 Biotecnica 和 Randox 检测之间观察到良好的一致性，但存在比例系统偏差。Biotecnica CRP 测定可提供可靠的犬血清 CRP 测量值，前提是样品不含干扰物。