当前位置: X-MOL 学术Int. J. Biol. Markers › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Up-regulated long non-coding RNA ILF3-AS1 indicates poor prognosis of nasopharyngeal carcinoma and promoted cell metastasis.
The International Journal of Biological Markers ( IF 2.3 ) Pub Date : 2020-09-22 , DOI: 10.1177/1724600820955199
Xuewen Yang 1 , Feng Lin 2 , Feng Gao 1
Affiliation  

Background:

Long non-coding RNAs (lncRNAs) have been confirmed to participate in the regulation of nasopharyngeal carcinoma. Here, we endeavored to explore the character of lncRNA ILF3-AS1 in the nasopharyngeal carcinoma and its function.

Methods:

A total of 68 nasopharyngeal carcinoma tissues and adjacent normal nasopharyngeal tissues were collected. Expressions of lncRNA ILF3-AS1 in these tissues were detected using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between the expression level of lncRNA ILF3-AS1 and clinical pathological characteristics was analyzed. Inhibition of lncRNA ILF3-AS1 was done using small interference RNA.

Results:

lncRNA ILF3-AS1 expression was significantly up-regulated in the 68 nasopharyngeal carcinoma tissue samples compared to their adjacent normal tissue samples. Increased lncRNA ILF3-AS1 level was related to the advanced tumor node metastasis stage and the metastasis of nasopharyngeal carcinoma. Also, increased lncRNA ILF3-AS1 indicated poor prognosis of nasopharyngeal carcinoma patients. Inhibition of lncRNA ILF3-AS1 reduced proliferation, invasion and migration of nasopharyngeal carcinoma cells. MicroRNA-320a (miR-320a) was determined as a direct target for lncRNA ILF3-AS1 in nasopharyngeal carcinoma. Furthermore, lncRNA ILF3-AS1 could sponge miR-320a to promote BMI1 expression. The expression of BMI1 was significantly inhibited by the down-regulation of lncRNA ILF3-AS1.

Conclusions:

For the first time, we demonstrated that lncRNA ILF3-AS1 was markedly over-expressed in nasopharyngeal carcinoma tissues and cells. Elevated lncRNA ILF3-AS1 expression was correlated with severe cancer stage and poor prognosis. lncRNA ILF3-AS1 could promote proliferation, invasion, and migration of cells, which might indicate a novel target site for the future diagnosis and therapy of nasopharyngeal carcinoma.



中文翻译:

上调的长链非编码RNA ILF3-AS1表明鼻咽癌预后不良并促进细胞转移。

背景:

长链非编码RNA(lncRNA)已被证实参与鼻咽癌的调控。在这里,我们努力探索lncRNA ILF3-AS1在鼻咽癌中的特征及其功能。

方法:

共收集鼻咽癌组织及邻近正常鼻咽组织68例。使用定量实时聚合酶链反应 (qRT-PCR) 检测这些组织中 lncRNA ILF3-AS1 的表达。分析lncRNA ILF3-AS1表达水平与临床病理特征的关系。使用小干扰 RNA 抑制 lncRNA ILF3-AS1。

结果:

与其相邻的正常组织样本相比,68 例鼻咽癌组织样本中 lncRNA ILF3-AS1 的表达显着上调。lncRNA ILF3-AS1水平升高与晚期肿瘤淋巴结转移分期和鼻咽癌转移有关。此外,增加的 lncRNA ILF3-AS1 表明鼻咽癌患者预后不良。lncRNA ILF3-AS1的抑制降低了鼻咽癌细胞的增殖、侵袭和迁移。MicroRNA-320a (miR-320a) 被确定为鼻咽癌中 lncRNA ILF3-AS1 的直接靶标。此外,lncRNA ILF3-AS1 可以海绵 miR-320a 以促进 BMI1 表达。lncRNA ILF3-AS1的下调显着抑制了BMI1的表达。

结论:

我们首次证明lncRNA ILF3-AS1在鼻咽癌组织和细胞中显着过表达。升高的 lncRNA ILF3-AS1 表达与严重的癌症分期和不良预后相关。lncRNA ILF3-AS1可以促进细胞的增殖、侵袭和迁移,这可能为未来鼻咽癌的诊断和治疗提供一个新的靶位点。

更新日期:2020-09-23
down
wechat
bug