Introduction. Freezing of ovarian tissue is used for preservation of fertility. The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis. Apoptosis is a complex process that has been studied in animal models. The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis. Methods. A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 μg/ml sodium selenite, through adding to the media) and control group. Vitrification-thawing process was done according to the existed protocols. Real-time PCR was used for gene expression study. The apoptosis gene profile included P53, Bax, Fas, and Bcl-2. General linear model was applied to study single gene associations and gene-gene interactions. Results. From the studied genes, P53 showed a significant downregulation in the selenium group in comparison to the control group (∆∆; ; ). Bcl-2 showed a significant upregulation in the selenium group in comparison to the control group (∆∆; ; ). No significant result was found for other genes. According to the multiple models, Bcl-2 showed a protective single gene association (; ), and FasBcl-2 interaction was significantly positive (; ). Conclusion. Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of P53 and upregulation of Bcl-2 at transcription level. Multivariable statistical models should be performed in future researches to study biological systems.

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硒对玻璃化冷冻后小鼠卵巢冷冻介质中凋亡相关基因表达的影响

简介。冷冻卵巢组织用于保存生育能力。冻融过程伴随着氧化应激和细胞凋亡的诱导。细胞凋亡是一个复杂的过程，已在动物模型中进行了研究。本研究旨在通过研究细胞凋亡相关基因的表达，探讨硒对小鼠卵巢玻璃化解冻过程中细胞凋亡的抑制作用，并设计单基因和基因作用的统计模型。抑制细胞凋亡的基因相互作用。方法。将来自 10 只小鼠的共 10 个右卵巢样本随机分为硒处理组（剂量 5  μg/ml 亚硒酸钠，通过添加到培养基中）和对照组。玻璃化解冻过程根据现有协议进行。实时荧光定量 PCR 用于基因表达研究。凋亡基因谱包括P53、Bax、Fas和Bcl-2。应用一般线性模型来研究单基因关联和基因-基因相互作用。结果。从所研究的基因来看，与对照组相比， P53在硒组中显示出显着的下调（ΔΔ; ; ）。 与对照组相比， Bcl-2在硒组中显示出显着上调（ΔΔ; ; ）。其他基因未发现显着结果。根据多个模型， Bcl-2显示出保护性单基因关联（; )， Fas Bcl -2相互作用显着阳性 (; ）。 结论。在玻璃化解冻过程的冷冻培养基中添加硒可以通过下调P53和上调Bcl-2在转录水平上减少小鼠卵巢冻融应激诱导的细胞凋亡在未来的研究中应该使用多变量统计模型来研究生物系统。