Introduction. The aim of this study was to investigate the ability of anti-bone morphogenetic protein 2 monoclonal antibody (anti-BMP-2 mAb) to functionalize scaffolds to mediate bone regeneration in a canine model. Materials and Methods. The mandibular right premolar 4 (PM4) was extracted in eight beagle dogs and grafted with anti-BMP-2 mAb+anorganic bovine bone mineral with 10% collagen (ABBM-C) and porcine bilayer native collagen membrane (CM). The ABBM-C and CM were functionalized with either anti-BMP-2 mAb (test group) or an isotype matched control mAb (control group). Animals were euthanized at 12 weeks for radiographic, histologic, and histomorphometric analyses. Outcomes were compared between groups. Results. 3D imaging using cone beam computed tomography (CBCT) revealed that sites treated with ABBM-C and CM functionalized with anti-BMP-2 mAb exhibited significantly more remaining bone width near the alveolar crest, as well as buccal bone height, compared with control groups. Histologic and histomorphometric analyses demonstrated that in anti-BMP-2 mAb-treated sites, total tissue volume was significantly higher in the coronal part of the alveolar bone crest compared with control sites. In anti-BMP-2 mAb-treated sites, bone formation was observed under the barrier membrane. Conclusion. Functionalization of the ABBM-C scaffold and CM appeared to have led to bone formation within healing alveolar bone sockets.

中文翻译：

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具有抗 BMP-2 单克隆抗体的功能化支架和屏障膜用于犬模型中的肺泡脊保存

简介。本研究的目的是研究抗骨形态发生蛋白 2 单克隆抗体（抗 BMP-2 mAb）在犬模型中使支架功能化以介导骨再生的能力。材料和方法。在八只比格犬中提取下颌右前磨牙 4 (PM4)，并移植抗 BMP-2 mAb + 具有 10% 胶原蛋白 (ABBM-C) 和猪双层天然胶原膜 (CM) 的无机牛骨矿物质。ABBM-C 和 CM 用抗 BMP-2 mAb（测试组）或同种型匹配的对照 mAb（对照组）功能化。在 12 周时对动物实施安乐死以进行放射照相、组织学和组织形态学分析。比较组间的结果。结果. 使用锥形束计算机断层扫描 (CBCT) 的 3D 成像显示，与对照组相比，用 ABBM-C 和用抗 BMP-2 mAb 功能化的 CM 治疗的部位在牙槽嵴附近表现出明显更多的剩余骨宽度以及颊侧骨高度. 组织学和组织形态学分析表明，在抗 BMP-2 mAb 治疗的部位，与对照部位相比，牙槽骨嵴冠状部分的总组织体积显着增加。在抗 BMP-2 mAb 处理的部位，在屏障膜下观察到骨形成。结论。ABBM-C 支架和 CM 的功能化似乎导致愈合牙槽骨窝内的骨形成。