The aim of this work was to enrich the knowledge on the potential applications of Elaeagnus mollis leaf extracts. For this purpose, the bioactive compounds (phenolic, flavonoid, alkaloid, proanthocyanidin, chlorophyll and carotene content), antioxidant activity, anti-HepG2 cell proliferation, and cholinesterase inhibitory potential (AChE and BChE) of E. mollis leaves which obtained from different habitats were quantitatively analyzed using various solvents (water, methanol, ethanol, and n-hexane). The results showed that the methanol extracts exhibited the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and the water extracts showed the best antioxidant activity in the 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging activity, ferric reducing antioxidant power (FRAP), and reducing power (RP) assays. Moreover, the methanol extracts showed the best inhibitory activity against cholinesterase and HepG2 cancer cells. Correlation analysis revealed that the high antioxidant and anti-HepG2 cell proliferation activities were mainly attributed to the total phenolics, flavonoids, and proanthocyanidins while AChE inhibition was attributed to the total alkaloid and carotene content. The statistical results showed that the effect of habitats was lower than that of different solvents used. Additionally, the metabolic profiles of E. mollis leaves were evaluated using HPLC-ESI-Q TRAP-MS/MS, and a total of 1,017 chemical components were detected and classified into 23 classes. The organic acids and derivatives ranked the first, followed by flavone, amino acid and derivatives, and so on. In conclusion, the effects of different solvents were more significant than the effects of different habitats and the methanol extracts of E. mollis leaves could be used as an effective source of functional active components, provide benefits to physical health care and be applied to the food and pharmaceutical industries.

这项工作的目的是丰富有关胡颓子叶提取物潜在应用的知识。为此，研究了大肠杆菌的生物活性化合物（酚类、类黄酮、生物碱、原花青素、叶绿素和胡萝卜素含量）、抗氧化活性、抗 HepG2 细胞增殖和胆碱酯酶抑制潜力（AChE 和 BChE）。使用不同溶剂（水、甲醇、乙醇和正己烷）对从不同栖息地获得的莫利斯叶子进行定量分析。结果表明,甲醇提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除活性最强,水提取物对2,2'-偶氮双-3-乙基苯并噻唑啉-6-的抗氧化活性最强。磺酸 (ABTS) 自由基清除活性、铁还原抗氧化能力 (FRAP) 和还原能力 (RP) 测定。此外，甲醇提取物对胆碱酯酶和HepG2癌细胞表现出最佳的抑制活性。相关分析显示，高抗氧化和抗HepG2细胞增殖活性主要归因于总酚、类黄酮和原花青素，而抑制AChE则归因于总生物碱和胡萝卜素含量。统计结果表明，生境的影响低于所用不同溶剂的影响。此外， E的代谢特征。采用 HPLC-ESI-Q TRAP-MS/MS 对mollis叶进行评价，共检测出 1,017 种化学成分，分为 23 类。有机酸及衍生物居首位，其次是黄酮、氨基酸及衍生物等。 总之，不同溶剂的影响比不同产地和大肠杆菌甲醇提取物的影响更显着。莫利斯叶可作为功能活性成分的有效来源，为身体保健提供益处，并可应用于食品和制药行业。