Wheat is a major food grain in Pakistan having a prominent role in agriculture as well as the economic status of the country. In the current study, seeds of 99 wheat landraces were characterized for the quantification of seed storage proteins (Albumins, Globulin, Gliadins, and Glutenin), enzyme activities of antioxidant enzymes i.e. Ascorbate peroxidase (APX), Catalase (CAT), Superoxide dismutase (SOD), Peroxidase (POD), one hydrolytic enzyme Protease (PROT) and non-enzymatic antioxidant enzyme Ascorbic acid (AsA). The landraces were categorized into low, medium, and high based on protein concentration and enzymes activities/content. The majority of the landraces were placed in the medium category. However, for the AsA parameter majority of the landraces were placed in the low category. The highest concentration of total extracted protein (184.88±0.7 mg/g. wt.), globulins (21.35±0.43 mg/g. wt.) and glutenin (20±0.04 mg/g. wt.) as well as the high activity of SOD (303±16.80 Units/g. wt.), and Ascorbic acid (533±36.1 Units/g. wt.) was identified in the wheat landrace “11757” collected from district Panjgur (Balochistan). The wheat landrace “11760”, collected from district Kech (Balochistan), contained the highest albumins concentration (65.42±0.02 mg/g. wt.) and highest activity for CAT (589.5±61.20 Units/g. wt.). The highest activity of POD (32341± 91.3) and PROT was observed in seeds of the wheat landrace “11618” collected from the Gilgit Baltistan region of Pakistan. The principal component analysis showed that the great variations existed for the tested parameters among the wheat landraces. The landraces with a high concentration of seed storage proteins and antioxidant enzyme activities can be used for breeding purposes to improve the nutrimental quality of wheat cultivars.

小麦是巴基斯坦的主要粮食作物，在农业和国家经济地位中发挥着重要作用。在当前的研究中，对 99 种小麦地方品种的种子进行了表征，以量化种子储存蛋白（白蛋白、球蛋白、麦醇溶蛋白和谷蛋白）、抗氧化酶（即抗坏血酸过氧化物酶 (APX)、过氧化氢酶 (CAT)、超氧化物歧化酶）的酶活性（ SOD）、过氧化物酶（POD）、一种水解酶蛋白酶（PROT）和非酶抗氧化酶抗坏血酸（AsA）。根据蛋白质浓度和酶活性/含量，地方品种被分为低、中和高。大多数地方品种都属于中等类别。然而，对于 AsA 参数，大多数地方品种都属于低类别。总提取蛋白（184.88±0.7 mg/g. wt.）、球蛋白（21.35±0.43 mg/g. wt.）和麦谷蛋白（20±0.04 mg/g. wt.）浓度最高且活性高在从旁吉古尔地区（俾路支省）收集的小麦地方品种“11757”中鉴定出 SOD（303±16.80 单位/克重量）和抗坏血酸（533±36.1 单位/克重量）。采自俾路支省凯赫地区的小麦地方品种“11760”含有最高的白蛋白浓度（65.42±0.02 毫克/克重量）和最高的 CAT 活性（589.5±61.20 单位/克重量）。在巴基斯坦吉尔吉特巴尔蒂斯坦地区采集的小麦地方品种“11618”的种子中观察到POD（32341±91.3）和PROT活性最高。主成分分析表明，小麦地方品种间测试参数存在较大变异。 种子贮藏蛋白和抗氧化酶活性高的地方品种可用于育种，提高小麦品种的营养品质。