Background: We developed a 3-dimensional (3D) culture system using a high-purity silica fiber scaffold of unwoven sheets called CellbedTM. Methods: We used adherent colon and esophagogastric junction adenocarcinoma cells, tongue squamous cell carcinoma (SqCC) cells, and nonadherent gastric cancer cells. These cells were subjected to staining with various substances and observed by electron microscopy. To evaluate the effects of extracellular matrix in carcinoma tissues, SqCC cells were cultured in Cellbed coated with collagens I, III, and IV. Results: Especially well-differentiated carcinoma cells cultured in this 3D system showed their own unique characteristics: luminal formation in adenocarcinoma cells and cell stratification and keratinization in SqCC cells. Scanning electron microscopy revealed the proliferation of cancer cells with cytoplasm entwined in Cellbed. Intercellular desmosomes in squamous epithelia were detected by transmission electron microscopy of vertical cross sections. SqCC cells cultured in Cellbed coated with collagen IV showed enhanced invasive and proliferative abilities. Conclusion: Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a “tissueoid cell culture system.” Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.

中文翻译：

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使用硅酸盐纤维支架的“类组织细胞培养系统”在癌症研究中的应用

背景：我们开发了一种 3 维 (3D) 培养系统，使用称为 CellbedTM 的无纺布片的高纯度二氧化硅纤维支架。方法：我们使用贴壁结肠和食管胃交界处腺癌细胞、舌鳞状细胞癌 (SqCC) 细胞和非贴壁胃癌细胞。这些细胞用各种物质染色并通过电子显微镜观察。为了评估细胞外基质在癌组织中的作用，在涂有胶原蛋白 I、III 和 IV 的细胞床中培养 SqCC 细胞。结果：特别是在该 3D 系统中培养的分化良好的癌细胞显示出其独特的特征：腺癌细胞中的管腔形成和 SqCC 细胞中的细胞分层和角化。扫描电子显微镜显示癌细胞增殖，细胞质缠绕在细胞床中。通过垂直横截面的透射电子显微镜检测鳞状上皮细胞间桥粒。在涂有胶原蛋白 IV 的 Cellbed 中培养的 SqCC 细胞显示出增强的侵袭和增殖能力。结论：由于在这个 3D 培养系统中培养的癌细胞的形态与活生物体中的相似，我们称该系统为“类组织细胞培养系统”。用胶原蛋白 IV 进行涂层可以改变细胞-基质相互作用以及体内微环境的重现。在涂有胶原蛋白 IV 的 Cellbed 中培养的 SqCC 细胞显示出增强的侵袭和增殖能力。结论：由于在这个 3D 培养系统中培养的癌细胞的形态与活生物体中的相似，我们称该系统为“类组织细胞培养系统”。用胶原蛋白 IV 进行涂层可以改变细胞-基质相互作用以及体内微环境的重现。在涂有胶原蛋白 IV 的 Cellbed 中培养的 SqCC 细胞显示出增强的侵袭和增殖能力。结论：由于在这个 3D 培养系统中培养的癌细胞的形态与活生物体中的相似，我们称该系统为“类组织细胞培养系统”。用胶原蛋白 IV 进行涂层可以改变细胞-基质相互作用以及体内微环境的重现。