The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

中文翻译：

---

新糖基化胶原蛋白：对神经母细胞瘤 F-11 细胞系的影响

神经系统的再生是一项具有挑战性的任务。目前，正在研究利用自然启发线索的再生医学方法，并且前景广阔。使用小寡糖和单糖功能化的基于蛋白质的基质的可能性令人感兴趣，因为这些可以微调以更好地模拟天然环境。胶原蛋白已被选为一种有前途的材料，它有可能进一步定制以掺入碳水化合物，以推动细胞行为向神经再生方向发展。事实上，证明将碳水化合物嫁接到胶原 2D 基质上可以增强其生物学意义。在本研究中，胶原 2D 基质被移植了不同的碳水化合物表位，并评估了它们驱动 F-11 神经母细胞瘤细胞向神经元分化的潜力。