The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device,ACS Central Science

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The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device
ACS Central Science ( IF 12.7 ) Pub Date : 2020-09-22 , DOI: 10.1021/acscentsci.0c00855
Alexander N Baker ₁ , Sarah-Jane Richards ₁ , Collette S Guy _{1,

2} , Thomas R Congdon ₁ , Muhammad Hasan ₁ , Alexander J Zwetsloot ₃ , Angelo Gallo ₁ , Józef R Lewandowski ₁ , Phillip J Stansfeld _{1,

2} , Anne Straube ₃ , Marc Walker ₄ , Simona Chessa ₅ , Giulia Pergolizzi ₅ , Simone Dedola ₅ , Robert A Field _{5,

6} , Matthew I Gibson _{1,

3}

Affiliation

There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.

中文翻译：

SARS-COV-2 刺突蛋白与唾液酸结合并能够在侧流护理诊断设备中进行快速检测

迫切需要了解新型冠状病毒 (SARS-COV-2)（COVID-19 的病原体）的行为，并开发即时诊断方法。在这里，糖纳米颗粒平台用于发现N-乙酰基神经氨酸对 SARS-COV-2 刺突糖蛋白具有亲和力，证明了其聚糖结合功能。粒径和涂层的优化使得能够在侧流中检测刺突糖蛋白，并显示出相对于 SARS-COV-1 刺突蛋白的选择性。使用病毒样颗粒和假型慢病毒模型，在 30 分钟内演示了纸质侧流检测，显示了该系统作为低成本检测平台的潜力。

更新日期：2020-11-25

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