Abstract

Antibacterial micron-sized cryogels, so-called microcryogels, were prepared by cryogelation of gelatin and integration of lysozyme. Gelation yield, specific surface area, macro-porosity and swelling degree of the microcryogels were examined in order to characterize their physical properties. MTT method was utilized to measure cell viability of the gelatin microcryogels with a period of 24, 48, and 72 h and no significant decrease was observed at 72 h. Apoptotic staining assay also showed high viability at 24, 48, 72 h in parallel with the control group. The antibacterial performances of the gelatin microcryogels against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli were examined. The results showed that the incorporation of lysozyme into gelatin microcryogels exhibited the antibacterial activity against S. aureus, B. subtilis, and E. coli, that may provide great potential for various applications in the biomedical industry.

摘要

抗菌微米级冷冻凝胶，即所谓的微冷冻凝胶，是通过明胶的冷冻凝胶化和溶菌酶的整合来制备的。检查了微冷冻凝胶的凝胶化产率、比表面积、大孔隙率和溶胀度，以表征它们的物理性质。MTT 方法用于测量明胶微冷冻凝胶的细胞活力，时间为 24、48 和 72 小时，在 72 小时未观察到显着降低。与对照组平行的细胞凋亡染色试验在 24、48、72 小时也显示出高活力。明胶微冻凝胶对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌的抗菌性能被审查。结果表明，将溶菌酶掺入明胶微凝胶中表现出对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的抗菌活性，为生物医学行业的各种应用提供了巨大的潜力。