ABSTRACT

Scaffold fabrication methods have a vital role in cartilage tissue engineering (CTE). For a long time, extracellular matrix (ECM) derivatives have been used to improve chondrogenesis and cartilage regeneration. In this study, polycaprolactone (PCL) scaffolds were prepared by 3D-printing and salt-leaching methods. Then, the prepared scaffolds were coated with fibrin and different acellular solubilised ECM concentrations. Adipose-derived stem cells (ADSCs) were added to fibrin/ECM hydrogel and seeded on the PCL scaffolds. Results showed that water absorption and compressive moduli of the 3D-printed scaffolds were higher than salt-leached scaffolds. As ECM was added and concentration increased, porosity was decreased and water uptake increased significantly. 3D-printed scaffolds with ECM2% group demonstrated a high compressive modulus and cell viability. Therefore, we concluded 2% is the most suitable concentration for acellular solubilised ECM in order to fabricate more biocompatible and applicable PCL/fibrin/ECM scaffolds in CTE.

摘要

支架制造方法在软骨组织工程 (CTE) 中起着至关重要的作用。长期以来，细胞外基质 (ECM) 衍生物已被用于改善软骨形成和软骨再生。在这项研究中，聚己内酯 (PCL) 支架是通过 3D 打印和盐浸法制备的。然后，制备的支架涂有纤维蛋白和不同的非细胞溶解 ECM 浓度。将脂肪干细胞 (ADSC) 添加到纤维蛋白/ECM 水凝胶中并接种在 PCL 支架上。结果表明，3D 打印支架的吸水率和压缩模量高于盐浸支架。随着 ECM 的加入和浓度的增加，孔隙度降低，吸水量显着增加。具有 ECM2% 组的 3D 打印支架表现出高压缩模量和细胞活力。因此，我们得出结论，2% 是脱细胞溶解 ECM 的最合适浓度，以便在 CTE 中制造更具生物相容性和适用性的 PCL/纤维蛋白/ECM 支架。