Long noncoding RNAs (lncRNAs), a group of transcripts, have been revealed to be critical participants in regulating multiple biological processes of malignant tumors. The knowledge of NPPA‐AS1 (a new lncRNA) in cancer research is hardly known. Thus, it is of urgent need to study the underlying role of NPPA antisense RNA 1 (NPPA‐AS1) in cervical cancer (CC). In this study, NPPA‐AS1 was discovered to be lowly expressed and upregulation of it impaired cell proliferation and migration in CC. Besides, downregulation of it led to opposite results. Molecular mechanism assays uncovered that increased expression of NPPA‐AS1 could inactivate Wnt/β‐catenin signaling pathway in CC. In addition, NPPA‐AS1 was found to negatively interact with miR‐302e whereas positively correlate with dickkopf‐1 (DKK1, an inhibitor of Wnt pathway) in CC. Besides, loss of function assay illuminated that miR‐302e inhibition restrained cell proliferation and migration in CC. Subsequent rescue assays confirmed that NPPA‐AS1 acted as a competing endogenous RNA in CC by sponging miR‐302e to upregulate DKK1 expression. Finally, the RE‐1 silencing transcription factor (REST) was testified to function as a transcription suppressor of NPPA‐AS1 in CC. In brief, REST‐repressed NPPA‐AS1 regulates CC progression by modulating miR‐302e/DKK1/Wnt/β‐catenin signaling pathway.

中文翻译：

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REST抑制的lncRNA NPPA-AS1通过调节miR-302e / DKK1 / Wnt /β-catenin信号通路调节宫颈癌的进展。

长的非编码RNA（lncRNA），一组转录物，已被证明是调节恶性肿瘤多种生物学过程的关键参与者。NPPA-AS1（一种新的lncRNA）在癌症研究中的知识鲜为人知。因此，迫切需要研究NPPA反义RNA 1（NPPA-AS1）在宫颈癌（CC）中的潜在作用。在这项研究中，发现NPPA-AS1的表达水平低，其上调会损害CC中的细胞增殖和迁移。此外，下调它导致相反的结果。分子机制分析发现，NPPA-AS1的表达增加可能使CC中的Wnt /β-catenin信号通路失活。此外，发现CC中NPPA-AS1与miR-302e负相关，而与dickkopf-1（DKK1，Wnt途径的抑制剂）正相关。除了，功能丧失试验表明，miR-302e抑制抑制了CC中细胞的增殖和迁移。随后的急救分析证实，NPPA-AS1通过海绵miR-302e上调DKK1表达，在CC中充当竞争性内源RNA。最后，证明RE-1沉默转录因子（REST）可以在CC中用作NPPA-AS1的转录抑制剂。简而言之，REST抑制的NPPA-AS1通过调节miR-302e / DKK1 / Wnt /β-catenin信号传导途径调节CC进程。RE-1沉默转录因子（REST）被证明可在CC中用作NPPA-AS1的转录抑制剂。简而言之，REST抑制的NPPA-AS1通过调节miR-302e / DKK1 / Wnt /β-catenin信号传导途径调节CC进程。RE-1沉默转录因子（REST）被证明可在CC中用作NPPA-AS1的转录抑制剂。简而言之，REST抑制的NPPA-AS1通过调节miR-302e / DKK1 / Wnt /β-catenin信号传导途径调节CC进程。