Disruption of glpF gene encoding the glycerol facilitator improves 1,3‐propanediol production from glucose via glycerol in Escherichia coli,Letters in Applied Microbiology

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Disruption of glpF gene encoding the glycerol facilitator improves 1,3‐propanediol production from glucose via glycerol in Escherichia coli
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-10-13 , DOI: 10.1111/lam.13391
R Sato _{1,

2} , T Tanaka ₁ , H Ohara ₁ , Y Aso _{1,

2}

Affiliation

Engineered Escherichia coli has recently been applied to produce 1,3‐propanediol (1,3‐PDO) from glucose. A metabolic intermediate in the production pathway, glycerol, is partially secreted into the extracellular of E. coli through a glycerol facilitator encoded by glpF, and this secretion consequently decreases 1,3‐PDO production. Therefore, we aimed to determine whether disrupting the glpF gene would improve 1,3‐PDO production in E. coli. The intracellular glycerol concentration in a glpF‐disruptant was 7·5 times higher than in a non‐disruptant. The glpF‐disrupted and non‐disrupted E. coli strains produced 0·26 and 0·09 g l−1 of 1,3‐PDO, respectively, from 1% glucose after 72 h of cultivation. The specific growth rate (μ) and the 1,3‐PDO yield from glucose (YP/S) in the disruptant were higher than those in the non‐disruptant (ΔglpF, μ = 0·08 ± 0·00 h−1, YP/S = 0·06 mol mol‐glucose−1; BW25113, μ = 0·06 ± 0·00 h−1, YP/S = 0·02 mol mol‐glucose−1). Disruption of the glpF gene decreased the production of the by‐product, acetic acid. These results indicated that disruption of glpF increased the intracellular concentration of glycerol and consequently increased 1,3‐PDO production in E. coli.

中文翻译：

编码甘油促进剂的 glpF 基因的破坏提高了大肠杆菌中通过甘油从葡萄糖中生产 1,3-丙二醇的能力

最近已应用工程大肠杆菌从葡萄糖生产 1,3-丙二醇 (1,3-PDO)。生产途径中的代谢中间体甘油通过由 glpF 编码的甘油促进剂部分分泌到大肠杆菌的细胞外，因此这种分泌减少了 1,3-PDO 的产生。因此，我们旨在确定破坏 glpF 基因是否会提高大肠杆菌中 1,3-PDO 的产量。glpF 干扰剂中的细胞内甘油浓度比非干扰剂高 7·5 倍。在培养 72 小时后，glpF 破坏和未破坏的大肠杆菌菌株分别从 1% 的葡萄糖中产生 0·26 和 0·09 gl-1 的 1,3-PDO。破坏剂中的比生长速率 (μ) 和来自葡萄糖的 1,3-PDO 产量 (YP/S) 高于非破坏剂 (ΔglpF, μ = 0·08 ± 0·00 h−1, YP/S = 0·06 mol mol-glucose−1; BW25113, μ = 0·06 ± 0·00 h−1, YP/S = 0·02 mol mol-glucose−1)。glpF 基因的破坏减少了副产物乙酸的产生。这些结果表明，glpF 的破坏增加了细胞内甘油的浓度，从而增加了大肠杆菌中 1,3-PDO 的产生。

更新日期：2020-10-13

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