A Belgium‐wide survey to determine the presence of ‘Candidatus Phytoplasma ulmi’ elm trees was performed on nearly 600 elm trees. The randomly sampled elms were screened for the presence of phytoplasmas using a standard generic nested PCR method. In five symptomless elm trees, phytoplasmas were detected. Multiple gene sequence analysis (16S rRNA, tuf, rplV‐rpsC‐rplP), virtual RFLP of the 16S rRNA gene fragment and phylogenetic analysis showed that the phytoplasma strains detected in all five elm trees could be classified into the 16SrXII‐E subgroup (‘Candidatus Phytoplasma fragariae’‐related phytoplasma). In addition, a maple tree (Acer pseudoplatanus) located in close proximity to one of these elm trees was also determined to be infected with ‘Ca. P. fragariae’. Ulmus and Acer are new host plant genera for this phytoplasma. Further studies are needed to elucidate the epidemiological impact of these findings.

中文翻译：

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一项植物质原体调查显示，榆树中存在'Candidatus Phytoplasma fragariae'。和比利时的Acer pseudoplatanus

一个比利时的调查，以确定“存在暂定已于近600榆树进行植原体红蜘蛛”榆树。使用标准的通用嵌套式PCR方法，对随机取样的榆树进行筛查，以检查是否存在植物浆体。在五棵无症状榆树中，检出了植原体。多基因序列分析（16S rRNA，tuf，rplV-rpsC-rplP），16S rRNA基因片段的虚拟RFLP以及系统发育分析表明，在所有五棵榆树中检测到的植物原质菌株都可以分为16SrXII-E亚组（'暂定植原体fragariae'相关的植原体）。此外，还确定位于其中一棵榆树附近的一棵枫树（Acer pseudoplatanus）感染了'钙。草莓。Ulmus和Acer是该植物质体的新寄主植物属。需要进一步的研究来阐明这些发现的流行病学影响。