In Vitro Biocompatibility of Diazirine-Grafted Biomaterials.,Macromolecular Rapid Communications

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In Vitro Biocompatibility of Diazirine-Grafted Biomaterials.
Macromolecular Rapid Communications ( IF 4.2 ) Pub Date : 2020-09-22 , DOI: 10.1002/marc.202000235
Ivan Djordjevic ₁ , Gautama Wicaksono ₁ , Ivan Solic ₁ , Terry W J Steele ₁

Affiliation

Photoactivation of aryl‐diazirines is an emerging method of rapid, covalent crosslinking under ambient conditions. These attributes make those compounds candidates for grafting onto inert polymer backbones in order to produce stimuli‐sensitive biomaterials. However, no risk assessments are available to gauge the toxicity of the leachable components after crosslinking activation. Herein, a stimuli‐sensitive biomaterial is formulated from diazirine‐grafted polycaprolactone tetrol. Also known as CaproGlu, this biomaterial undergoes UVA‐activated crosslinking, with many positive attributes toward bioadhesive applications; hydrophobic, solvent‐free, liquid at room temperature, and transitions into a foam biorubber after mild UVA illumination. As a model diazirine‐grafted biomaterial, hydrolyzed CaproGlu leachates are evaluated for genotoxicity and skin sensitization, namely, Ames test, direct peptide reactivity, and ARE‐Nrf2 luciferase assays. The degradation products of diazirine‐mediated crosslinking observe little to no risk of in vitro genotoxicity or skin sensitization.

中文翻译：

叠氮基接枝生物材料的体外生物相容性。

芳基二嗪的光活化是在环境条件下快速共价交联的新兴方法。这些特性使这些化合物成为接枝到惰性聚合物主链上的候选物，以生产对刺激敏感的生物材料。但是，没有风险评估可用于评估交联活化后可浸出组分的毒性。在这里，一种刺激敏感的生物材料是由重氮基接枝的聚己内酯四氢萘制成的。这种生物材料也称为CaproGlu，它经过UVA活化的交联，对生物粘合剂的应用具有许多积极的特性。疏水，无溶剂，在室温下为液体，在温和的UVA照射后转变为泡沫生物橡胶。作为模型的二嗪类生物材料移植，评估水解的CaproGlu浸出液的遗传毒性和皮肤致敏性，即Ames试验，直接肽反应性和ARE-Nrf2荧光素酶测定。二嗪嗪介导的交联降解产物几乎没有或没有体外遗传毒性或皮肤过敏的风险。

更新日期：2020-11-06

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