Present study investigated the Cu²⁺ removal potential of Trichoderma lixii CR700, isolated from enormously heavy metal polluted electroplating wastewater. In the batch study, actively growing CR700 was able to remove 84.6% of Cu²⁺ at the concentration 10 mg/L of Cu²⁺ within 120 h after incubation and the accumulated and surface adsorbed amount of Cu was 0.51 and 0.47 mg/g of dry biomass respectively. T. lixii CR700 also showed efficient Cu²⁺ removal potential in the pH ranges from 5.0 to 8.0, in the presence of other co-occurring contaminant such as heavy metal, anions and metabolic inhibitor as well from real tannery wastewater. Alteration on cell surface of Cu²⁺ treated mycelia of T. lixii CR700 was analyzed using scanning electron microscope. Fourier transform infrared spectroscopic analysis was performed to identify the role of surface functional group in Cu²⁺ adsorption which revealed that COO^─ functional group lead Cu²⁺ adsorption onto the surface of T. lixii CR700. Thus, T. lixii CR700 uses simultaneous surface sorption and accumulation mechanism in Cu²⁺ removal and can be potentially applied for bioremediation of Cu²⁺ contaminated wastewater in ecofriendly, safe and sustainable way.

本研究调查了从重金属污染严重的电镀废水中分离出来的木霉菌CR700的Cu ²⁺去除潜力。在分批研究中，积极生长的CR700在温育后120 h内能够以10 mg / L的Cu ²⁺浓度去除84.6％的Cu ²⁺，并且Cu的累积和表面吸附量分别为0.51和0.47 mg / g干生物质。在存在其他共生污染物（例如重金属，阴离子和代谢抑制剂）以及制革厂废水中同时存在的条件下，T。lixii CR700在pH范围为5.0至8.0时也显示出有效的Cu ²⁺去除潜力。Cu ^2+的细胞表面变化使用扫描电子显微镜分析处理的螺旋藻CR700的菌丝体。傅里叶变换红外分光分析进行，以确定在Cu中的表面官能团的作用²⁺吸附这表明，COO ^─官能团导致的Cu ²⁺吸附到表面T. lixii CR700。因此，T。lixii CR700利用同时的表面吸附和累积机制去除Cu ^2+，并有可能以生态友好，安全和可持续的方式应用于Cu ²⁺污染废水的生物修复。