The development of phytopharmaceuticals for the treatment of diseases such as cancer is a necessity, and its development requires to establish the conditions of commercial cultivation of the plant. Asclepias subulata has exhibited potent antiproliferative activity due to its cardenolide glycoside content. This work aimed to determine the cardenolide profile and antiproliferative activity of ethanolic extracts of A. subulata obtained from an experimental crop under different water stress treatments. Asclepias subulata was germinated in a shade house and was transplanted to the field in spring under different conditions: drought (D), drought with hydrogel (DG), drought with double row (DD), irrigation (I), irrigation with hydrogel (IG), and irrigation with double row (ID). For 12 months, biomass, concentration of calotropin and corotoxigenin 3-O-glucopyranoside, and antiproliferative activity were evaluated. The results showed the lower biomass for D treatments (283 g plant^-1) while the I treatments showed the highest biomass (831 g plant^-1). In general, the concentration of cardenolides was higher under drought treatments: for corotoxigenin 3-O-glucopyranoside, it was 530 μg g^-1 while for calotropin, it was 204 μg g^-1 dry weight. The ethanolic extracts showed strong antiproliferative activity and selectivity in the A549 cell line (human alveolar carcinoma). The D treatment showed an IC₅₀ <2 μg mL^-1 while in ARPE-19 (retinal pigment epithelia cell line), it was IC₅₀ (the half maximal inhibitory concentration)> 200 μg mL^-1. Based on findings in the present work, it can be concluded that A. subulata can be cultivated with a high production of the active compounds. This work represents an important advance in the studies for the potential development of a phytopharmaceutical based on A. subulata.

开发用于治疗诸如癌症的疾病的植物药物是必要的，并且其发展需要建立植物的商业栽培条件。马利福禄表现出了强大的抗增殖活性，由于其cardenolide苷含量。这项工作的目的是确定cardenolide轮廓和乙醇提取物的抗增殖活性A.福禄从不同水分胁迫处理的实验作物获得。枯草菌在阴凉的房子里发芽，并在不同条件下于春季移植到田间：干旱（D），水凝胶干旱（DG），双行干旱（DD），灌溉（I），水凝胶灌溉（IG），和双排灌溉（ID）。在12个月的时间里，评估了生物量，促钙素和Corotoxigenin 3-O-吡喃葡萄糖苷的浓度以及抗增殖活性。结果表明，D处理的生物量较低（283 g植物^-1），而I处理的生物量最高（831 g植物^-1）。一般而言，干旱处理下心内酯的浓度较高：对于Corotoxigenin 3-O-吡喃葡萄糖苷，为530μgg ^-1，而对于calotropin，为204μgg ^-1净重。乙醇提取物在A549细胞系（人肺泡癌）中显示出强大的抗增殖活性和选择性。D处理显示IC ₅₀ < 2μgmL ^-1，而在ARPE-19（视网膜色素上皮细胞系）中，IC _50为（最大最大抑制浓度的一半）> 200μgmL ^-1。基于本发明的发现，可以得出结论，可以以高产量的活性化合物来培养枯草曲霉。这项工作代表了在研究基于sub.subulata的植物药的潜在发展方面的重要进展。