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Response of microalgae Chlorella vulgaris to Cr stress and continuous Cr removal in a membrane photobioreactor
Chemosphere ( IF 8.1 ) Pub Date : 2020-09-23 , DOI: 10.1016/j.chemosphere.2020.128422
Miao-Miao Lu , Feng Gao , Chen Li , Hong-Li Yang

This study evaluated the toxicity of Cr(VI) to microalgae Chlorella vulgaris, and its removal by continuous microalgae cultivation in membrane photobioreactor (MPBR). Batch cultivation in photobioreactors showed that low concentration of Cr(VI) (0.5 and 1.0 mg L-1) stimulated the growth of C. vulgaris, while 2.0 and 5.0 mg L-1 Cr(VI) in the wastewater significantly inhibited the growth of C. vulgaris. Superoxide dismutase and catalase activities that represented cellular antioxidant capacity significantly increased at 0.5 and 1.0 mg L-1 Cr(VI), and then gradually decreased with the continuous increase of Cr(VI) concentration. The content of malondialdehyde, which represents the degree of cellular oxidative damage, increased with the increase of Cr(VI) concentration and reached the peak value at 2.0 mg L-1 Cr(VI). C. vulgaris was then cultured in MPBR equipped with hollow-fiber ultrafiltration membrane module to achieve continuous removal of Cr from wastewater. With the in-situ solid-liquid separation function of the membrane module, solid retention time (SRT) and hydraulic retention time (HRT) of the reactor could be controlled separately. Experimental results showed that both SRT and HRT had significant effects on the algal biomass production and pollutants removal. During the continuous operation, MPBR achieved a maximum total Cr reduction of 50.0% at HRT of 3-day and SRT of 40-day, and a maximum volumetric removal rate of total Cr of 0.21 mg L-1 d-1 at HRT of 2-day and SRT of 40-day.



中文翻译:

膜光生物反应器中微藻小球藻对Cr胁迫和连续Cr去除的响应

这项研究评估了六价铬对微藻小球藻的毒性,并通过在膜光生物反应器(MPBR)中连续微藻培养去除了六价铬。在光生物反应器中分批培养表明,低浓度的Cr(VI)(0.5和1.0 mg L -1)刺激了寻常梭菌的生长,而废水中的2.0和5.0 mg L -1 Cr(VI)显着抑制了C.的生长。C.寻常型。代表细胞抗氧化能力的超氧化物歧化酶和过氧化氢酶活性在0.5和1.0 mg L -1时显着增加Cr(VI),然后随着Cr(VI)浓度的不断增加而逐渐降低。丙二醛的含量,代表细胞氧化损伤的程度,随Cr(VI)浓度的增加而增加,并在2.0 mg L-1 Cr(VI)达到峰值。寻常念珠菌然后在配有中空纤维超滤膜组件的MPBR中进行培养,以实现废水中Cr的连续去除。利用膜组件的原位固液分离功能,可以分别控制反应器的固体保留时间(SRT)和水力保留时间(HRT)。实验结果表明,SRT和HRT对藻类生物量的产生和污染物的去除均具有显着影响。在连续操作,MPBR实现了在3天的水力停留时间最大总Cr的还原的50.0%和40天SRT的,和总Cr的最大体积去除速率0.21毫克的L的-1 d -1以2 HRT天和40天的SRT。

更新日期:2020-09-23
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