Sesamum indicum is an important oilseed crop with beneficiary nutrients such as essential fatty acids, proteins, and lignan. S. indicum crop improvement using conventional breeding methods was disastrous. Moreover, no reproducible regeneration and transformation protocol for large-scale generation of independent primary transformed plant lines of S. indicum virtually exists. Therefore, in the present study, a reproducible regeneration and transformation method was developed for S. indicum. Direct shoot regeneration (94.44%) was achieved using plumule tips as an explant source and when maintained on regeneration medium (BM11) an amalgamation of half the strength of MS macronutrients and B5 macronutrients together with MS micronutrients and vitamins. Maximum number of shoots (~ 16–19 shoots/explant) was obtained on BM11 medium supplemented with TDZ (1 mg/L) and BAP (0.1 mg/L). High root induction efficiency (~ 97%) was observed on RM4 medium consisted of half MS macronutrients, SH micronutrients and vitamins. The developed protocol was found effective for other commercially valuable genotypes such as Co 1, Phule til and Tilottama as well. Successful regeneration was subsequently extended to transformation of reporter gene (gfp: green fluorescent protein) as revealed by molecular analyses includes Southern hybridization, northern hybridization, real time PCR and histological study of the transformants developed in the present study. Based on the Southern analysis the calculated transformation frequency was found to be 1.33%. The presently developed regeneration and transformation system might facilitate any desired improvement of sesame crop.

芝麻是一种重要的油料作物，具有有益的营养成分，例如必需脂肪酸，蛋白质和木脂素。小号。使用常规育种方法改良标记作物是灾难性的。而且，实际上不存在用于大规模产生印度钩球菌的独立初级转化植物系的可再现的再生和转化方案。因此，在本研究中，开发了一种可复制的印度链霉菌再生和转化方法。。使用胚芽尖端作为外植体来源可实现直接苗芽再生（94.44％），当维持在再生培养基（BM11）上时，MS大量营养素和B5大量营养素以及MS微量营养素和维生素的强度降低一半。在补充了TDZ（1 mg / L）和BAP（0.1 mg / L）的BM11培养基上获得最大芽数（〜16-19芽/植株）。在由一半MS大量营养素，SH微量营养素和维生素组成的RM4培养基上观察到较高的根诱导效率（约97％）。发现已开发的方案对其他商业有价值的基因型（例如Co 1，Phule til和Tilottama）也有效。成功的再生随后扩展到了报道基因的转化（gfp：通过分子分析揭示的绿色荧光蛋白）包括Southern杂交，Northern杂交，实时PCR和本研究开发的转化体的组织学研究。根据Southern分析，计算出的转化频率为1.33％。当前开发的再生和转化系统可以促进芝麻作物的任何期望的改良。