Fungal endophytes, both mycorrhizal and non-mycorrhizal, are involved in the development of the life cycle of orchids, providing potential beneficial relationships. Here, we assess the succession of changes in the diversity of fungal symbionts associated with a terrestrial temperate orchid species, Anacamptis morio subsp. champagneuxii, over three phenological stages: developed leaves but no stem elongation, flowering, and fruiting. Fungi endophyte associated with roots were obtained by culture in sterile conditions. A total of 18 morphotypes—one Mortierellomycota, two Basidiomycota and 15 Ascomycota—were differentiated, and were also characterized using PCR and DNA sequencing techniques. Only three of the 18 OTUs are shared among the three phenological stages examined: Westerdykella sp., a member of Ceratobasidiaceae, and Fusarium oxysporum, representing a relative abundance of between 28% (fruiting) to 41% (flowering). Our research confirmed that fungal symbionts varied among the different phenological stages examined, the peak of endophyte diversity appearing in the flowering stage. The availability of a diverse mycobiota seems to be important for the survival of orchid plants because it may cover particular physiological needs, and knowledge concerning this mycobiota is of special relevance in the establishment of reliable conservation programmes.

菌根的和非菌根的真菌内生菌都参与了兰花生命周期的发展，提供了潜在的有益关系。在这里，我们评估与陆生温带兰花物种Anacamptis morio亚种相关的真菌共生体多样性的连续变化。香槟色，在三个物候阶段：叶发达，但没有茎伸长，开花和结果。通过在无菌条件下培养获得与根相关的内生真菌。总共分化了18种形态型，一种是Mortierellomycota，两种Basidiomycota和15种Ascomycota，并且还使用PCR和DNA测序技术对其进行了表征。在所研究的三个物候阶段中，只有18个OTU中的三个被共享：Westerdykella sp。（Ceratobasidiaceae的成员）和Fusarium oxysporum的相对丰度在28％（结果）到41％（开花）之间。我们的研究证实，真菌共生体在所研究的不同物候阶段之间存在差异，内生菌多样性的高峰出现在开花期。多样化的分枝杆菌的可用性对于兰花植物的生存似乎很重要，因为它可能满足特殊的生理需要，有关此分枝杆菌的知识与建立可靠的保护计划特别相关。