Analysis of wolves dietary is a currently important theme because of the discussion about wolves preying on livestock as sheep or goats. We developed molecular markers to especially amplify the DNA of the prey out of wolf scat. For this purpose, we used the mitochondrial D-Loop using public available sequences for wolf and seven potential prey species (even-toed ungulates). We developed special primers amplifying either the wolves DNA or the prey DNA. In a fragment of 223-225 basepairs (bp) length we identified 21 SNPs, two 1-bp indels and one 3-bp indel, and three microsatellites to separate seven prey species from each other. Validation of the markers was performed by sequencing the PCR products of 12 fresh prey tissues and 20 wolf scat samples using the different primer pairs.

对狼的饮食进行分析是当前重要的主题，因为有关狼以绵羊或山羊为食的牲畜的讨论。我们开发了分子标记，特别是从狼粪中扩增出猎物的DNA。为此，我们使用了针对狼和七个潜在猎物（偶蹄类有蹄类动物）的公共序列使用的线粒体D环。我们开发了特殊的引物，可扩增狼DNA或猎物DNA。在一个长度为223-225个碱基对（bp）的片段中，我们鉴定出21个SNP，两个1 bp插入缺失和一个3 bp插入缺失以及三个微卫星，从而将七个猎物物种彼此分离。通过使用不同的引物对对12个新鲜猎物组织和20只狼粪样品的PCR产物进行测序，对标记物进行验证。