The saccharification of Chlorella sp. biomass by single and combined commercial carbohydrase enzymes, Termamyl SC, Dextrozyme GA, and Cellic CTec2, was investigated. A substrate concentration of 100-g volatile solids (VS)/L was optimal for all the enzymes used. The optimal enzyme dosages for Termamyl SC, Dextrozyme GA, and Cellic CTec2 were 160-units/g VS, 320-units/g VS, and 40–filter paper units/g VS, respectively. The maximum reducing sugar yield, 130-mg/g VS, was obtained with a Dextrozyme GA treatment, which is equivalent to 38.37% hydrolysis efficiency based on carbohydrate content. A combined-enzyme treatment with Cellic CTec2, Termamyl SC, and Dextrozyme GA raised the reducing sugar yield and hydrolysis efficiency to 186.69-mg/g VS and 54.85%, respectively. Scanning and transmission electron microscopic images indicated morphological alterations in Chlorella sp. cells as an effect of enzyme treatment. Enzyme treatment improved the hydrogen production, hydrogen production rate, and hydrogen yield from Chlorella sp. biomass. The maximum hydrogen yield, 42.24-mL/g VS_added, was obtained from a Dextrozyme GA–hydrolyzed microalgae biomass. This was 82.46% higher than that obtained from untreated biomass (23.15-mL H₂/g VS_added). Fourier-transform infrared analysis revealed that the functional groups of Chlorella sp. biomass, especially carbohydrates, were modified by both enzymatic treatment and the hydrogen fermentation process. This work demonstrated the efficient saccharification of Chlorella sp. biomass using combined cell wall– and starch-degrading enzymes. The results also elucidate that the pretreatment of Chlorella sp. biomass by commercial glucoamylase is a promising approach to solubilize internal starch granules and promote biohydrogen production without the addition of any cell wall–degrading enzyme.

小球藻的糖化sp。研究了单一和组合的商业糖酶，Termamyl SC，右旋酶GA和Cellic CTec2产生的生物量。对于所有使用的酶，最佳的底物浓度为100 g挥发性固体（VS）/ L。Termamyl SC，Dextrozyme GA和Cellic CTec2的最佳酶剂量分别为160单位/克VS，320单位/克VS和40滤纸单位/克VS。通过Dextrozyme GA处理获得的最大还原糖产量为130 mg / g VS，基于碳水化合物含量，这相当于38.37％的水解效率。用Cellic CTec2，Termamyl SC和Dextrozyme GA联合酶处理可将还原糖产量和水解效率分别提高至186.69-mg / g VS和54.85％。扫描和透射电镜图像显示了形态变化小球藻藻。细胞作为酶处理的作用。酶处理提高了小球藻的产氢量，产氢率和产氢量。生物质。最大氢收率，42.24毫升/克VS_添加，是从Dextrozyme GA-水解微藻生物质中获得。这比从未经处理的生物量（23.15-毫升水获得了更高的82.46％₂ / g的VS_添加）。傅里叶变换红外分析表明小球藻属的功能基团。生物质，特别是碳水化合物，通过酶处理和氢发酵过程进行了改性。这项工作证明了小球藻的有效糖化作用sp。结合细胞壁和淀粉降解酶的生物质。结果还阐明了小球藻的预处理。商业化的葡糖淀粉酶产生的生物质是一种有前途的方法，可以溶解内部淀粉颗粒并促进生物氢的产生，而无需添加任何可降解细胞壁的酶。