Abstract

Application of natural colors in food and medicine, due to toxic effects of synthetic colors has been considered recently. Phycocyanin is regarded as the most preferred phycobilin pigment of cyanobacteria Spirulina because it has medical and medicinal features. In current study, phycocyanin was extracted and purified from Spirulina platensis using 5 freezing/thawing cycles, ultrasonic disruption, ammonium sulphate precipitation and dialysis. Purified phycocyanin was tested by UV-spectrophotometrically and using FT-IR and HPLC methods. The antifungal effect of purified phycocyanin was evaluated versus Candida albicans on sabouraud dextrose agar plates, and maximum anticandidal activity was revealed in the range from 20 to 25 mg/mL of phycocyanin. Making sure of phycocyanin’s antifungal effect, the efficacy of its produced cream (using 1.5 and 3% of phycocyanin compared to control) was investigated on mice infected with C. albicans. Based on obtained results, those groups of mice treated by formulation containing phycocyanin improved much sooner than control group.

中文翻译：

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螺旋藻藻蓝蛋白产生的局部乳膏可加速白色念珠菌感染小鼠的伤口愈合。

摘要

由于合成色的毒性作用，最近已经考虑将天然色应用于食品和药品中。藻蓝蛋白被认为是蓝藻螺旋藻中最优选的藻蓝蛋白色素，因为它具有医学和医学特性。在目前的研究中，藻蓝蛋白提取和纯化从螺旋藻 螺旋藻使用5-冷冻/解冻循环，超声波破碎，硫酸铵沉淀和透析。通过紫外分光光度法并使用FT-IR和HPLC方法测试纯化的藻蓝蛋白。相对于白色念珠菌，评估了纯化藻蓝蛋白的抗真菌作用在sabouraud葡萄糖琼脂平板上，在20至25 mg / mL的藻蓝蛋白中显示出最大的抗candidal活性。为了确定藻蓝蛋白的抗真菌作用，在感染白色念珠菌的小鼠上研究了其乳膏的功效（与对照相比使用了1.5％和3％的藻蓝蛋白）。根据获得的结果，用含藻蓝蛋白的制剂治疗的小鼠组比对照组早得多的改善。